TY - JOUR
T1 - Partial purification of toxins Hl1, Hl2 and Hl3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida: Vejovidae)
AU - Escobar, Enrique
AU - Rivera, Carlos
AU - Tincopa, Luz
AU - Rivera, Dani
PY - 2002/1/1
Y1 - 2002/1/1
N2 - © 2002 Facultad de Ciencias Biológicas UNMSM. The proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1, Hl2 y Hl3; which immobilize, respectively, insects (Grillus sp.), crustaceans (Porcellio laevis) and the inoculated limb of white mice. The three toxins are basic proteins and have no proteolitic or phospholipase activity. In addition, Hl3 increases the plasmatic level of creatine kinase after its inoculation in the gastrocnemius muscle of mice. By PAGE-SDS, Hl3 shows only one protein band of 12,5 KDa, while the other toxins have protein contaminants.
AB - © 2002 Facultad de Ciencias Biológicas UNMSM. The proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1, Hl2 y Hl3; which immobilize, respectively, insects (Grillus sp.), crustaceans (Porcellio laevis) and the inoculated limb of white mice. The three toxins are basic proteins and have no proteolitic or phospholipase activity. In addition, Hl3 increases the plasmatic level of creatine kinase after its inoculation in the gastrocnemius muscle of mice. By PAGE-SDS, Hl3 shows only one protein band of 12,5 KDa, while the other toxins have protein contaminants.
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M3 - Article
SN - 1561-0837
SP - 3
EP - 10
JO - Revista Peruana de Biologia
JF - Revista Peruana de Biologia
ER -