Pathogenicity of a Yersinia ruckeri strain from an outbreak of yersiniosis in rainbow trout (Oncorhynchus mykiss) from Huaraz, Peru

V. Fernando Mesías, L. Mario Vargas, Q. Alexander Cueva, S. Alberto Manchego, C. Nieves Sandoval

Research output: Contribution to journalArticle

Abstract

© 2019 Universidad Nacional Mayor de San Marcos. All rights reserved. The objectives of this study were to determine the pathogenicity of a strain of Yersinia ruckeri, from an outbreak of yersiniosis in Huaraz and to characterize histopathological lesions in various organs of rainbow trout fingerlings (Oncorhynchus mykiss). One hundred fingerlings with an average weight of 7.25 g were used, divided into 5 groups of 20 individuals (four experimental and one control), kept in 30 L ponds. Experimental groups were inoculated via i.m. with 0.1 ml of the bacterial strain in concentrations of 1x104/ml (G1), 4x104/ml (G2), 1x108/ml (G3) and 4x108/ml (G4), and the control group with PBS. Clinical signs, external and internal lesions, morbidity and mortality were recorded for 12 days post-inoculation. Samples of liver, intestine, spleen, kidney, muscle and gills were collected for histopathological analysis. All fish inoculated with Y. ruckeri showed signs and lesions compatible with yersiniosis. Most of individuals had inappetence and melanosis, as well as splenomegaly, mucous content in the intestine and intestinal congestion. Mortalities were 65 and 60% for G1 and G2, respectively and 100% for G3 and G4. Fat degeneration was found in liver, necrosis in intestine and muscle, presence of melanomacrophages and congestion in spleen and kidney, hyperplasia and fusion of lamellae in gills. Results indicate that Y. ruckeri strain from Huaraz is highly pathogenic.
Original languageAmerican English
Pages (from-to)387-403
Number of pages17
JournalRevista de Investigaciones Veterinarias del Peru
DOIs
StatePublished - 1 Jan 2019

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