The aim of this study was to evaluate phagocytosis stimulation in Oncorhynchus mykiss (rainbow trout) juveniles immunized with outer membrane fraction (OMF) from Flavobacterium psychrophilum USM-3 native strain and challenged with Flavobacterium psychrophilum 1947T standard strain. Fifteen specimens were immunized intraperitoneally with 10 μg of OMF and fifteen specimens with 0.1ml of bidistilled water. Afterwards, both groups were challenged with Flavobacterium psychrophilum 1947T standard strain. After 21 days from first immunization, phagocytic activity and index were determined. Mean phagocytic activity in immunized fish was 22.4% mean while in control group it was 0.5% (p<0.05). Phagocytic index percentage in immunized fish and control group was 32.5% and 0.5% (p<0.05) respectively. It was concluded that outer membrane fraction from Flavobacterium psychrophilum USM-3 native strain stimulates phagocytosis in Oncorhynchus mykiss juveniles infected with F. psychrophilum 1947T.
|Translated title of the contribution||Phagocytosis stimulation in Oncorhynchus mykiss juveniles immunized with outer membrane fractions from a Flavobacterium psychrophilum native strain|
|Journal||Revista Electronica de Veterinaria|
|State||Published - Jan 2013|