Fenotipo y genotipo del virus de la diarrea viral aislado de bovinos en el perú

Translated title of the contribution: Phenotype and genotype of bovine viral diarrhoea virus isolated in peruvian cattle

R. Mariluz Araínga, G. Hermelinda Rivera, G. Juan Carlos Huamán, S. Alberto Manchego

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The aim of this study was to determine the phenotype and genotype of Bovine Viral Diarrhoea Virus (BVDV) in Peruvian cattle. During the period 1998-2007, tissues of aborted foetus, blood, serum or plasma samples of bovine with acute infection or persistently infected from dairy herds of various locations in the country were collected and the BVDV antigen was detected by immnunofluorescence or capture ELISA tests, and positive samples were inoculated en BVDV free BT cells and cultured until third passage to determine the biotype. Both animal tissues and third passage of positive samples were tested by a Real Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for genotyping the strains. Forty one BVDV strains were isolated in BT cells. Eighty five percent (35/41) of the isolates were non-cytopathic and 14.6% (6/41) cytophatic. Forty five and a half percent (25/55) and 74.5% (41/55) of the animal tissues and from the 3rd passage samples respectively, were positive to BVDV by Real Time RT-PCR test. All isolates belonged to BVDV-1 genotype. These results contribute to a better understanding of the epidemiology of the bovine viral diarrhoea disease in Peru and suggest that BVDV-2 is absent or has low prevalence in dairy herds.

Translated title of the contributionPhenotype and genotype of bovine viral diarrhoea virus isolated in peruvian cattle
Original languageSpanish
Pages (from-to)192-203
Number of pages12
JournalRevista de Investigaciones Veterinarias del Peru
Volume21
Issue number2
StatePublished - 2010

Bibliographical note

Funding Information:
a) Transcripción Reversa (Síntesis de ADNc). Para la Transcripción Reversa se utilizó el kit DyNAmo SYBR Green 2-step qRT-PCR F430L (Finnzymes, Fin- landia) que contiene los reactivos nece-sarios para la síntesis de ADNc y para la amplificación del ADN (PCR) por los primers específicos. Para la síntesis del ADNc se preparó la mezcla de reactivos que contiene el kit en un volumen de 20 µl al cual se le añadió el ARN extraído y purificado colocándose en el equipo termociclador PTC 200 (Peltier Therme Cycler) Chromo 4 (detector continuo de fluorescencia) (MJ Research, UK) por 10 minutos a 25 °C, 30 minutos a 37 °C, 5 minutos a 85 °C , y una vez finalizado se mantuvo a 4 °C.

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