Phytochemical screening by LC-ESI-MS/MS and effect of the ethyl acetate fraction from leaves and stems of jatropha macrantha müll arg. On ketamine-induced erectile dysfunction in rats

Johnny Aldo Tinco-Jayo, Enrique Javier Aguilar-Felices, Edwin Carlos Enciso-Roca, Jorge Luis Arroyo-Acevedo, Oscar Herrera-Calderon

Research output: Contribution to journalArticlepeer-review

Abstract

Jatropha macrantha Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditional medicine as an aphrodisiac and erectile dysfunction (ED). The aim of this study was to determine the phytochemical constituents in leaves and stems ethyl acetate fraction (LEAF and SEAF) of J. macrantha and to compare the antioxidant activity and the ameliorative effect on ketamine-induced erectile dysfunction in rats. The phytochemical constituents were determined by LC-ESI-MS/MS, the total phenolic compounds and total flavonoids (TPC and TF) by Folin-Ciocalteu and aluminum chloride, respectively. The antioxidant activity was determined by DPPH, ABTS, and FRAP assays. Experimental groups were divided as follows: I: negative control; II: positive control (ketamine at 50 mg/ kg/d); III: sildenafil 5 mg/kg; IV, V, VI: LEAF at 25, 50 and 100 mg/kg, respectively, and VII, VIII, IX: SEAF at 25, 50, and 100 mg/kg, respectively. The phytochemical analysis revealed the presence mainly of coumarins, flavonoids, phenolic acids, and terpenes. TPC of LEAF and SEAF were 359 ± 5.21 mg GAE/g and 306 ± 1.93 mg GAE/g, respectively; TF in LEAF and SEAF were 23.7 ± 0.80 mg EQ/g, and 101 ± 1.42 mg EQ/g, respectively. The DPPH, ABTS, FRAP in SEAF were 647 ± 3.27; 668 ± 2.30; and 575 ± 2.86 µmol TE/g, respectively, whilst LEAF showed 796 ± 3.15; 679 ± 0.85; and 806 ± 3.42 µmol TE/g, respectively. Regarding sexual behavior, LEAF showed a better effect in mount frequency, intromission frequency, ejaculation frequency, mount latency, intromission latency, ejaculatory latency, and post ejaculatory latency than SEAF. As conclusion, LEAF of J. macrantha at 50 mg/kg showed a better effect on sexual behavior in male rats with erectile dysfunction than SEAF but not higher than sildenafil.

Original languageEnglish
Article number115
JournalMolecules
Volume27
Issue number1
DOIs
StatePublished - 1 Jan 2022
Externally publishedYes

Bibliographical note

Funding Information:
This research was funded by the Fondo Nacional de Desarrollo Cient?fico, Tecnol?gico y de Innovaci?n Tecnol?gico (FONDECYT, Peru), grant number 389-2019-FONDECYT.

Funding Information:
Funding: This research was funded by the Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológico (FONDECYT, Peru), grant number 389-2019-FONDECYT.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Antioxidant agent
  • Aphrodisiac
  • Experimental model
  • Huanarpo
  • LC-MS plant extract
  • Liquid chromatography
  • Sexual behavior

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