Pre- and postzygotic isolation and Haldane rule effects in reciprocal crosses of Danaus erippus and Danaus plexippus (Lepidoptera: Danainae), supported by differentiation of cuticular hydrocarbons, establish their status as separate species

Mirian M. Hay-Roe, Gerardo Lamas, James L. Nation

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Hybridization experiments and cuticular hydrocarbon analyses were performed to determine the extent of the reproductive isolation present to clarify the taxonomic status of Danaus erippus (Cramer, 1775), which has often been classified as a subspecies of Danaus plexippus (Linnaeus, 1758). The results of crosses between D. erippus and Danaus plexippus nigrippus (Haensch, 1909) showed pre- and postzygotic isolation. Pupal inviability, and Haldane rule effects (male hybrids only, the homogametic sex in butterflies) were observed after the crosses. These results reinforce the hypothesis that D. plexippus and D. erippus are separate, reproductively isolated species. However, we have also investigated the cuticular hydrocarbons in D. erippus and D. p. nigrippus with temperature-programmed gas chromatography. The chromatographic profiles of peaks were similar in both taxa, with retention indices in the range 2100-3797. The profiles were characterized by the presence of 15 peaks whose retention indices were in the range 2501-3772. Although no major qualitative differences between the two species were observed, we did find some quantitative differences. A principal component analysis showed a clear separation between members of D. erippus and D. p. nigrippus, suggesting that semiochemical differences might have become differentiated after isolation, perhaps in response to different physiological pressures.
Original languageAmerican English
Pages (from-to)445-453
Number of pages9
JournalBiological Journal of the Linnean Society
Volume91
Issue number3
DOIs
StatePublished - 1 Jul 2007

Keywords

  • Cuticular hydrocarbons
  • Danaus plexippus nigrippus
  • Gas chromatography
  • Hybridization
  • Monarch butterflies
  • Peru
  • Postzygotic isolation
  • Prezygotic isolation

Fingerprint Dive into the research topics of 'Pre- and postzygotic isolation and Haldane rule effects in reciprocal crosses of Danaus erippus and Danaus plexippus (Lepidoptera: Danainae), supported by differentiation of cuticular hydrocarbons, establish their status as separate species'. Together they form a unique fingerprint.

Cite this