Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic Campylobacter Strains Isolated from Chickens in Retail Markets of Lima, Peru

Christian Benites, Diego Anampa, Domingo Torres, Ivette Avalos, Miguel Rojas, Carlos Conte, César Lázaro

Research output: Contribution to journalArticlepeer-review

Abstract

Background: In this study, we aimed to estimate the prevalence, tetracycline resistance and presence of Tet(O) in Campylobacter strains isolated from chicken in markets of Lima, Peru. Methods: A total of 250 chicken samples were obtained from traditional markets (skin, n = 120) and supermarkets (meat, n = 130). Samples were subjected to microbiological assays for identification of Campylobacter spp. according to ISO 10272-2017, and the isolates were then submitted to species identification by PCR. Phenotypic resistance to tetracyclines was assessed by the Kirby–Bauer test, and the presence of the Tet(O) gene was determined by PCR. Results: A significantly higher prevalence (p < 0.0001) of Campylobacter coli in skin samples from traditional markets (97.5%) than in meat samples from supermarkets (36.2%) was observed. On the other hand, Campylobacter jejuni was confirmed only in 3.1% of meat samples. All Campylobacter species isolated from skin and meat samples were phenotypically resistant to tetracyclines; however, the presence of the Tet(O) gene in C. coli was identified in 76.9% and 66.0% of skin and meat samples, no significant statistical difference (p = 0.1488) was found between these prevalence. All C. jejuni isolated from chicken meat samples from supermarkets were positive for Tet(O) gene. Conclusions: This study confirms the high prevalence of C. coli isolated from chicken sold in traditional markets and supermarkets in Lima, Peru, and in more than 70% of these strains, phenotypic resistance to tetracyclines could be linked with expression of the Tet(O) gene. It is necessary to evaluate other genes involved in resistance to tetracyclines and other groups of antibiotics in campylobacter strains isolated from chicken meat.

Original languageEnglish
Article number1580
JournalAntibiotics
Volume11
Issue number11
DOIs
StatePublished - Nov 2022
Externally publishedYes

Bibliographical note

Funding Information:
This research was funded by PROCIENCIA—CONCYTEC within the framework of the call “Proyecto Investigación Básica 2019-01”, [Contract number 405-2019-FONDECYT]; the Vice-Rectorate for Research and Postgraduate Studies—Universidad Nacional Mayor de San Marcos in the framework of the call “Proyectos de Investigación con Financiamiento para Grupos de Investigación” [Code: A21080431] and “Programa de Proyectos Interdisciplinarios para el Fomento de la Cooperación Interinstitucional en Investigación e Innovación—Política Nacional de Educación Superior Técnico Productiva 2021 [Code: A2108001i].

Publisher Copyright:
© 2022 by the authors.

Keywords

  • antibiotic resistance
  • Campylobacter
  • chicken
  • tetracyclines

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