Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens

Charles G. Gauci; César M. Jayashi; Armando E. Gonzalez; Julia Lackenby; Marshall W. Lightowlers

doi:10.1016/j.vaccine.2012.04.019

Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens

Charles G. Gauci, César M. Jayashi, Armando E. Gonzalez, Julia Lackenby, Marshall W. Lightowlers

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding protein (MBP). Groups of pigs were immunized twice with the GST fusions of the antigens and boosted a third time with the MBP fusions prior to receiving a challenge infection with T. solium eggs. The TSOL16 antigen was found to be capable of inducing high levels of immunity in pigs against a challenge infection with T. solium. Immunological investigations identified differences in immune responses in the pigs vaccinated with the various antigens. The results demonstrate that the TSOL16 antigen could be a valuable adjunct to current porcine vaccination approaches and may allow the further development of new vaccination strategies against T. solium cysticercosis.

Original language	English
Pages (from-to)	3824-3828
Number of pages	5
Journal	Vaccine
Volume	30
Issue number	26
DOIs	https://doi.org/10.1016/j.vaccine.2012.04.019
State	Published - 6 Jun 2012
Externally published	Yes

Bibliographical note

Funding Information:
Assistance with statistical analyses by Garry Anderson is gratefully acknowledged. Funding was from the Wellcome Trust, Animal Health in the Developing World grant 075818 and the Australian National Health and Medical Research Council , grants 350279 , 400109 and 628320 .

Keywords

Antigen
Cysticercosis
Parasite
Recombinant
Taenia
Vaccine

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10.1016/j.vaccine.2012.04.019

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title = "Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens",

abstract = "Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding protein (MBP). Groups of pigs were immunized twice with the GST fusions of the antigens and boosted a third time with the MBP fusions prior to receiving a challenge infection with T. solium eggs. The TSOL16 antigen was found to be capable of inducing high levels of immunity in pigs against a challenge infection with T. solium. Immunological investigations identified differences in immune responses in the pigs vaccinated with the various antigens. The results demonstrate that the TSOL16 antigen could be a valuable adjunct to current porcine vaccination approaches and may allow the further development of new vaccination strategies against T. solium cysticercosis.",

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note = "Funding Information: Assistance with statistical analyses by Garry Anderson is gratefully acknowledged. Funding was from the Wellcome Trust, Animal Health in the Developing World grant 075818 and the Australian National Health and Medical Research Council , grants 350279 , 400109 and 628320 .",

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AU - Lightowlers, Marshall W.

N1 - Funding Information: Assistance with statistical analyses by Garry Anderson is gratefully acknowledged. Funding was from the Wellcome Trust, Animal Health in the Developing World grant 075818 and the Australian National Health and Medical Research Council , grants 350279 , 400109 and 628320 .

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N2 - Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding protein (MBP). Groups of pigs were immunized twice with the GST fusions of the antigens and boosted a third time with the MBP fusions prior to receiving a challenge infection with T. solium eggs. The TSOL16 antigen was found to be capable of inducing high levels of immunity in pigs against a challenge infection with T. solium. Immunological investigations identified differences in immune responses in the pigs vaccinated with the various antigens. The results demonstrate that the TSOL16 antigen could be a valuable adjunct to current porcine vaccination approaches and may allow the further development of new vaccination strategies against T. solium cysticercosis.

AB - Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding protein (MBP). Groups of pigs were immunized twice with the GST fusions of the antigens and boosted a third time with the MBP fusions prior to receiving a challenge infection with T. solium eggs. The TSOL16 antigen was found to be capable of inducing high levels of immunity in pigs against a challenge infection with T. solium. Immunological investigations identified differences in immune responses in the pigs vaccinated with the various antigens. The results demonstrate that the TSOL16 antigen could be a valuable adjunct to current porcine vaccination approaches and may allow the further development of new vaccination strategies against T. solium cysticercosis.

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KW - Parasite

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KW - Taenia

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Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens

Abstract

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Keywords

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