Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens

Charles G. Gauci, César M. Jayashi, Armando E. Gonzalez, Julia Lackenby, Marshall W. Lightowlers

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding protein (MBP). Groups of pigs were immunized twice with the GST fusions of the antigens and boosted a third time with the MBP fusions prior to receiving a challenge infection with T. solium eggs. The TSOL16 antigen was found to be capable of inducing high levels of immunity in pigs against a challenge infection with T. solium. Immunological investigations identified differences in immune responses in the pigs vaccinated with the various antigens. The results demonstrate that the TSOL16 antigen could be a valuable adjunct to current porcine vaccination approaches and may allow the further development of new vaccination strategies against T. solium cysticercosis.

Original languageEnglish
Pages (from-to)3824-3828
Number of pages5
JournalVaccine
Volume30
Issue number26
DOIs
StatePublished - 6 Jun 2012

Bibliographical note

Funding Information:
Assistance with statistical analyses by Garry Anderson is gratefully acknowledged. Funding was from the Wellcome Trust, Animal Health in the Developing World grant 075818 and the Australian National Health and Medical Research Council , grants 350279 , 400109 and 628320 .

Keywords

  • Antigen
  • Cysticercosis
  • Parasite
  • Recombinant
  • Taenia
  • Vaccine

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