Bothrops brazili is a pitviper from Amazonian region, responsible for many accidents in Peru. Despite its relevance, its venom has not been extensively characterized. In the present work, Bothrops brazili venom (BbV) components were analyzed by RP-HPLC, SDS-PAGE and MALDI-TOF/TOF. Approximately 37 proteins were identified, belonging to 7 families. Snake venom metalloproteinases (SVMPs) were the most abundant proteins of the venom (33.05%), followed by snake venom serine proteinases (SVSPs, 26.11%), phospholipases A2 (PLA2, 25.57%), snake C-type lectins (CTLs, 9.61%), L-aminoacid oxidase (LAAO, 3.80%), cystein-rich secretory proteins (CRISP, 1.67%) and Bradykinin-potentiating peptide (BPP, 0.20%). In vitro enzymatic activities of BbV showed high levels of SVMP activity and reduced Hyal activity in comparison with other bothropic venoms. Furthermore, BbV reduced VERO cells viability. ELISA and Western Blotting showed that both Peruvian and Brazilian bothropic antivenoms were able to recognize BbV components. This work provides an overview of BbV venom content and indicates a potential efficiency of Peruvian and Brazilian antivenoms to treat accidents with this species.
Bibliographical noteFunding Information:
The authors acknowldge the ?Laborat?rio Multiusu?rio de Prote?mica (LMProt) ? Centro de Laborat?rios Multiusu?rios (CELAM), ICB-UFMG? for technical support in the mass spectrometry analysis. This research was supported by the Conselho Nacional de Desenvolvimento Cient??co e Tecnol?gico, Brazil (CNPq) (Process: 406163/2018-9), Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, Brazil - CAPES (Program COFECUB; Process: 88881.191812/2018-01) and by Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG; Process: PPM-00270-16).
This research was supported by the Conselho Nacional de Desenvolvimento Cientíﬁco e Tecnológico , Brazil ( CNPq ) (Process: 406163/2018-9 ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior , Brazil - CAPES (Program COFECUB; Process: 88881.191812/2018-01 ) and by Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil ( FAPEMIG; Process: PPM-00270-16 ).
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- Amazonian region
- Bothropic venoms
- Bothrops brazili
- Proteomic analyses