Proteomic study of activated Taenia solium oncospheres

S. J. Santivañez, A. Hernández-González, N. Chile, A. Oleaga, Y. Arana, S. Palma, M. Verastegui, A. E. Gonzalez, R. Gilman, H. H. Garcia, M. Siles-Lucas

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Taenia solium cysticerci are a major cause of human seizures and epilepsy in the world. In the gastrointestinal tract of infected individuals, taeniid eggs release the oncospheres, which are then activated by intestinal stimuli, getting ready to penetrate the gut wall and reach distant locations where they transform in cysticerci. Information about oncospheral molecules is scarce, and elucidation of the oncosphere proteome could help understanding the host-parasite relationship during the first steps of infection. In this study, using liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis, we could identify a set of oncospheral proteins involved in adhesion, protein folding, detoxification and proteolysis, among others. In addition, we have characterized one of the identified molecules, the parasite 14-3-3, by immunoblot and immunolocalization. The identification of these oncospheral proteins represents the first step to elucidate their specific roles in the biology of the host-parasite relationship.

Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume171
Issue number1
DOIs
StatePublished - May 2010

Bibliographical note

Funding Information:
This project was partially funded by the Fogarty International Center/NIH (training grants D43 TW001140 , TW007490 and TW006581 ), and the Bill and Melinda Gates Foundation (training grant #33848 ). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the sponsors. AHG acknowledges financial support from the CSIC-Predoctorate JAE Spanish grant programme.

Keywords

  • Activated
  • Oncospheres
  • Proteome
  • Taenia solium

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