Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru

Tito Sánchez-Rojas; Abraham Espinoza-Culupú; Pablo Ramírez; Leo Kei Iwai; Fabio Montoni; Diego Macedo-Prada; Marcos Sulca-López; Yerson Durán; Mariella Farfán-López; Jennifer Herencia

doi:10.3390/microorganisms10102002

Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru

Tito Sánchez-Rojas, Abraham Espinoza-Culupú, Pablo Ramírez, Leo Kei Iwai, Fabio Montoni, Diego Macedo-Prada, Marcos Sulca-López, Yerson Durán, Mariella Farfán-López, Jennifer Herencia

Research output: Contribution to journal › Article › peer-review

Abstract

Mine tailings are produced by mining activities and contain diverse heavy metal ions, which cause environmental problems and have negative impacts on ecosystems. Different microorganisms, including yeasts, play important roles in the absorption and/or adsorption of these heavy metal ions. This work aimed to analyze proteins synthesized by the yeast Yarrowia lipolytica AMJ6 (Yl-AMJ6), isolated from Andean mine tailings in Peru and subjected to stress conditions with common heavy metal ions. Yeast strains were isolated from high Andean water samples impacted by mine tailings from Yanamate (Pasco, Peru). Among all the isolated yeasts, the Yl-AMJ6 strain presented LC50 values of 1.06 mM, 1.42 mM, and 0.49 mM for the Cr⁺⁶, Cu⁺², and Cd⁺² ions, respectively. Proteomic analysis of theYl-AMJ6 strain under heavy metal stress showed that several proteins were up- or downregulated. Biological and functional analysis of these proteins showed that they were involved in the metabolism of proteins, nucleic acids, and carbohydrates; response to oxidative stress and protein folding; ATP synthesis and ion transport; membrane and cell wall; and cell division. The most prominent proteins that presented the greatest changes were related to the oxidative stress response and carbohydrate metabolism, suggesting the existence of a defense mechanism in these yeasts to resist the impact of environmental contamination by heavy metal ions.

Original language	English
Article number	2002
Journal	Microorganisms
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/microorganisms10102002
State	Published - Oct 2022
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Ministerio de la Producción (grants 391 PNICP-PIAP-2014 Innóvate Perú); Vicerrectorado de Investigación y Posgrado (VRIP) from Universidad Nacional Mayor de San Marcos (grant B17100046a); Grants 2013/07467-1 and 2017/17943-6 from the São Paulo Research Foundation (FAPESP), Brazil and Vicerrectorado de Investigación, Fiscal Year 2022 from Universidad Señor de Sipán, Peru (Support of grammar english correction and payment of publication fees).

Publisher Copyright:
© 2022 by the authors.

Keywords

Andean mining
heavy metal ions
oxidative stress
proteomics
Yarrowia lipolytica

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10.3390/microorganisms10102002

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Sánchez-Rojas, T., Espinoza-Culupú, A., Ramírez, P., Iwai, L. K., Montoni, F., Macedo-Prada, D., Sulca-López, M., Durán, Y., Farfán-López, M., & Herencia, J. (2022). Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru. Microorganisms, 10(10), [2002]. https://doi.org/10.3390/microorganisms10102002

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title = "Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru",

abstract = "Mine tailings are produced by mining activities and contain diverse heavy metal ions, which cause environmental problems and have negative impacts on ecosystems. Different microorganisms, including yeasts, play important roles in the absorption and/or adsorption of these heavy metal ions. This work aimed to analyze proteins synthesized by the yeast Yarrowia lipolytica AMJ6 (Yl-AMJ6), isolated from Andean mine tailings in Peru and subjected to stress conditions with common heavy metal ions. Yeast strains were isolated from high Andean water samples impacted by mine tailings from Yanamate (Pasco, Peru). Among all the isolated yeasts, the Yl-AMJ6 strain presented LC50 values of 1.06 mM, 1.42 mM, and 0.49 mM for the Cr+6, Cu+2, and Cd+2 ions, respectively. Proteomic analysis of theYl-AMJ6 strain under heavy metal stress showed that several proteins were up- or downregulated. Biological and functional analysis of these proteins showed that they were involved in the metabolism of proteins, nucleic acids, and carbohydrates; response to oxidative stress and protein folding; ATP synthesis and ion transport; membrane and cell wall; and cell division. The most prominent proteins that presented the greatest changes were related to the oxidative stress response and carbohydrate metabolism, suggesting the existence of a defense mechanism in these yeasts to resist the impact of environmental contamination by heavy metal ions.",

keywords = "Andean mining, heavy metal ions, oxidative stress, proteomics, Yarrowia lipolytica",

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year = "2022",

month = oct,

doi = "10.3390/microorganisms10102002",

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Sánchez-Rojas, T, Espinoza-Culupú, A, Ramírez, P, Iwai, LK, Montoni, F, Macedo-Prada, D, Sulca-López, M, Durán, Y, Farfán-López, M & Herencia, J 2022, 'Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru', Microorganisms, vol. 10, no. 10, 2002. https://doi.org/10.3390/microorganisms10102002

TY - JOUR

T1 - Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru

AU - Sánchez-Rojas, Tito

AU - Espinoza-Culupú, Abraham

AU - Ramírez, Pablo

AU - Iwai, Leo Kei

AU - Montoni, Fabio

AU - Macedo-Prada, Diego

AU - Sulca-López, Marcos

AU - Durán, Yerson

AU - Farfán-López, Mariella

AU - Herencia, Jennifer

PY - 2022/10

Y1 - 2022/10

N2 - Mine tailings are produced by mining activities and contain diverse heavy metal ions, which cause environmental problems and have negative impacts on ecosystems. Different microorganisms, including yeasts, play important roles in the absorption and/or adsorption of these heavy metal ions. This work aimed to analyze proteins synthesized by the yeast Yarrowia lipolytica AMJ6 (Yl-AMJ6), isolated from Andean mine tailings in Peru and subjected to stress conditions with common heavy metal ions. Yeast strains were isolated from high Andean water samples impacted by mine tailings from Yanamate (Pasco, Peru). Among all the isolated yeasts, the Yl-AMJ6 strain presented LC50 values of 1.06 mM, 1.42 mM, and 0.49 mM for the Cr+6, Cu+2, and Cd+2 ions, respectively. Proteomic analysis of theYl-AMJ6 strain under heavy metal stress showed that several proteins were up- or downregulated. Biological and functional analysis of these proteins showed that they were involved in the metabolism of proteins, nucleic acids, and carbohydrates; response to oxidative stress and protein folding; ATP synthesis and ion transport; membrane and cell wall; and cell division. The most prominent proteins that presented the greatest changes were related to the oxidative stress response and carbohydrate metabolism, suggesting the existence of a defense mechanism in these yeasts to resist the impact of environmental contamination by heavy metal ions.

AB - Mine tailings are produced by mining activities and contain diverse heavy metal ions, which cause environmental problems and have negative impacts on ecosystems. Different microorganisms, including yeasts, play important roles in the absorption and/or adsorption of these heavy metal ions. This work aimed to analyze proteins synthesized by the yeast Yarrowia lipolytica AMJ6 (Yl-AMJ6), isolated from Andean mine tailings in Peru and subjected to stress conditions with common heavy metal ions. Yeast strains were isolated from high Andean water samples impacted by mine tailings from Yanamate (Pasco, Peru). Among all the isolated yeasts, the Yl-AMJ6 strain presented LC50 values of 1.06 mM, 1.42 mM, and 0.49 mM for the Cr+6, Cu+2, and Cd+2 ions, respectively. Proteomic analysis of theYl-AMJ6 strain under heavy metal stress showed that several proteins were up- or downregulated. Biological and functional analysis of these proteins showed that they were involved in the metabolism of proteins, nucleic acids, and carbohydrates; response to oxidative stress and protein folding; ATP synthesis and ion transport; membrane and cell wall; and cell division. The most prominent proteins that presented the greatest changes were related to the oxidative stress response and carbohydrate metabolism, suggesting the existence of a defense mechanism in these yeasts to resist the impact of environmental contamination by heavy metal ions.

KW - Andean mining

KW - heavy metal ions

KW - oxidative stress

KW - proteomics

KW - Yarrowia lipolytica

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U2 - 10.3390/microorganisms10102002

DO - 10.3390/microorganisms10102002

M3 - Artículo

AN - SCOPUS:85140910778

SN - 2076-2607

VL - 10

JO - Microorganisms

JF - Microorganisms

IS - 10

M1 - 2002

ER -

Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru

Abstract

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Keywords

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