Purification and partial characterization of a toxin (Hm3) from Hadruroides mauryi (Francke y Soleglad, 1980) (Scorpiones, Iuridae) venom

Luz Velásquez, Enrique Escobar

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

© 2004 Facultad de Ciencias Biológicas UNMSM. A toxin (Hm3) was purified from the scorpion venom Hadruroides mauryi, by ion-exchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7. The toxin produce contraction and immobilize the inoculated limb of white mice, and is a basic protein with a molecular weight of 4,5 kDa. After of 45 minutes inoculated in gastrocnemius muscle of white mice, Hm3 (30,4 μg) increases the plasmatic level of creatine kinase from 252,6 UI/L to 3779,3 UI/L and lactate deshidrogenase from 142,7 UI/L to 248,2 UI/L; likewise increases the level of intramuscular calcium from 34,1 nmoles to 69,3 nmoles. This toxin have not proteolitic or phospholipase activity.
Original languageAmerican English
Pages (from-to)153-160
Number of pages8
JournalRevista Peruana de Biologia
StatePublished - 1 Jan 2004

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