Refrigeración de semen canino utilizando glucosa, fructosa, trehalosa o sacarosa para prolongar la supervivencia espermática

Translated title of the contribution: Refrigeration of canine semen using glucose, fructose, trehalose or sucrose to extend sperm survival

A. Jonathan Flores, A. Víctor Fernández, U. Héctor Huamán, Luis Felipe Ruiz Garcia, Alexei Vicent Santiani Acosta

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The effect of glucose, fructose, trehalose, and sucrose, as components of the extender, was evaluated on canine sperm viability during refrigeration at 5 ºC. The spermatic fraction of 16 dog ejaculations were distributed in 5 aliquots containing an extender based on Tris – citric acid – egg yolk, but differing in the presence of one of the sugars, plus a control without sugar. The five treatments were evaluated after 24, 48, 72, and 96 hours of refrigeration in relation to progressive motility and functional integrity of the membrane by the hypoosmotic swelling test (HOS). All groups, with the exception of the control group, the sperm motility maintained within acceptable ranges during the study (90% on day 0 and 60% on day 4). The integrity of the membrane remained slightly superior in groups with sugar (87-90%) in comparison with the control group (83-85%). The glucose and sucrose showed the best results in motility and HOS. It is concluded that no significant damage occurred on the sperm membrane while keeping semen at 5 ºC, and that the use of any of the tested sugars as energy-giving substrate for the sperm cells (especially glucose and sucrose) is of great importance for the maintenance of appropriate levels of sperm motility and functional integrity of the membrane.

Translated title of the contributionRefrigeration of canine semen using glucose, fructose, trehalose or sucrose to extend sperm survival
Original languageSpanish
Pages (from-to)26-34
Number of pages9
JournalRevista de Investigaciones Veterinarias del Peru
Volume21
Issue number1
StatePublished - 2010
Externally publishedYes

Bibliographical note

Funding Information:
Se empleó el método de manipulación digital, consistente en aplicar masajes suaves y de manera alternada sobre el cuerpo del pene del animal ejerciendo una ligera presión sobre el bulbo del pene cada tres segundos, hasta lograr una erección parcial; luego se retrae el prepucio y se sujeta el bulbo con la mano enguantada, ejerciendo una constante presión sobre el mismo, para lograr una total turgencia y marcado movimiento o reflejo pélvico; y cuando el perro levanta alguna de sus extremidades posteriores, se dirige el pene hacia atrás (rotación de 180 grados) para co-lectar el eyaculado.

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