Rotavirus A, C, and H in Brazilian pigs: potential for zoonotic transmission of RVA

Patrícia S. Flores; Fábio B. Costa; Ariane R. Amorim; Gabriella S. Mendes; Miguel Rojas; Norma Santos

doi:10.1177/1040638720967673

Rotavirus A, C, and H in Brazilian pigs: potential for zoonotic transmission of RVA

Patrícia S. Flores, Fábio B. Costa, Ariane R. Amorim, Gabriella S. Mendes, Miguel Rojas, Norma Santos

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Rotaviruses (RVs) have been identified as one of the main infectious causes of diarrhea in young pigs. We determined the prevalence of rotavirus A (RVA), C (RVC), and H (RVH) in pigs on a Brazilian farm. Samples were screened by reverse-transcription (RT)-PCR, and samples positive for RVA were genotyped by PCR amplification and sequencing analysis. Of the 329 fecal samples analyzed, 102 (30.9%) were positive for RV, 25 (7.6%) contained RVA only, 32 (9.7%) contained RVC only, and 31 (9.4%) contained RVH only. Co-circulation, the presence of ≥ 2 RVs in a sample, was detected in 14 (4.2%) samples. Of the 15 animals with diarrhea, 6 (40%) were positive for RV, and of the 314 asymptomatic animals, 96 (30.6%) were positive for RV; there was no statistically significant difference between the 2 groups (p = 0.441). Genotyping of RVA strains showed co-circulation of genotypes G1, G3, G9-P[8]-I1, and I2-E1. Phylogenetic analysis showed that some of the RVA genotypes found in pigs had high percentages of identity when compared with reference strains from humans, which suggests interspecies transmission. Because RVs may be zoonotic, excretion of RVs into the environment can result in transmission to agricultural workers causing interspecies infections and allowing the emergence of new reassorted viruses.

Original language	English
Pages (from-to)	129-135
Number of pages	7
Journal	Journal of Veterinary Diagnostic Investigation
Volume	33
Issue number	1
DOIs	https://doi.org/10.1177/1040638720967673
State	Published - Jan 2021
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq grants 404984/2018-5 and 301469/2018-0), the Funda??o Carlos Chagas de Amparo ? Pesquisa do Estado do Rio de Janeiro, Brazil (FAPERJ, grant E-26/202.909/2017), and Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES; Finance Code 001). The funders were not involved in the study design, data collection, data interpretation, or the decision to submit the work for publication.

Funding Information:
This study was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq grants 404984/2018-5 and 301469/2018-0), the Fundação Carlos Chagas de Amparo à Pesquisa do Estado do Rio de Janeiro, Brazil (FAPERJ, grant E-26/202.909/2017), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Finance Code 001). The funders were not involved in the study design, data collection, data interpretation, or the decision to submit the work for publication.

Keywords

epidemiology
genotyping
rotavirus
swine
viral diarrhea
zoonosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "Rotaviruses (RVs) have been identified as one of the main infectious causes of diarrhea in young pigs. We determined the prevalence of rotavirus A (RVA), C (RVC), and H (RVH) in pigs on a Brazilian farm. Samples were screened by reverse-transcription (RT)-PCR, and samples positive for RVA were genotyped by PCR amplification and sequencing analysis. Of the 329 fecal samples analyzed, 102 (30.9%) were positive for RV, 25 (7.6%) contained RVA only, 32 (9.7%) contained RVC only, and 31 (9.4%) contained RVH only. Co-circulation, the presence of ≥ 2 RVs in a sample, was detected in 14 (4.2%) samples. Of the 15 animals with diarrhea, 6 (40%) were positive for RV, and of the 314 asymptomatic animals, 96 (30.6%) were positive for RV; there was no statistically significant difference between the 2 groups (p = 0.441). Genotyping of RVA strains showed co-circulation of genotypes G1, G3, G9-P[8]-I1, and I2-E1. Phylogenetic analysis showed that some of the RVA genotypes found in pigs had high percentages of identity when compared with reference strains from humans, which suggests interspecies transmission. Because RVs may be zoonotic, excretion of RVs into the environment can result in transmission to agricultural workers causing interspecies infections and allowing the emergence of new reassorted viruses.",

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AU - Rojas, Miguel

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AB - Rotaviruses (RVs) have been identified as one of the main infectious causes of diarrhea in young pigs. We determined the prevalence of rotavirus A (RVA), C (RVC), and H (RVH) in pigs on a Brazilian farm. Samples were screened by reverse-transcription (RT)-PCR, and samples positive for RVA were genotyped by PCR amplification and sequencing analysis. Of the 329 fecal samples analyzed, 102 (30.9%) were positive for RV, 25 (7.6%) contained RVA only, 32 (9.7%) contained RVC only, and 31 (9.4%) contained RVH only. Co-circulation, the presence of ≥ 2 RVs in a sample, was detected in 14 (4.2%) samples. Of the 15 animals with diarrhea, 6 (40%) were positive for RV, and of the 314 asymptomatic animals, 96 (30.6%) were positive for RV; there was no statistically significant difference between the 2 groups (p = 0.441). Genotyping of RVA strains showed co-circulation of genotypes G1, G3, G9-P[8]-I1, and I2-E1. Phylogenetic analysis showed that some of the RVA genotypes found in pigs had high percentages of identity when compared with reference strains from humans, which suggests interspecies transmission. Because RVs may be zoonotic, excretion of RVs into the environment can result in transmission to agricultural workers causing interspecies infections and allowing the emergence of new reassorted viruses.

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Rotavirus A, C, and H in Brazilian pigs: potential for zoonotic transmission of RVA

Abstract

Bibliographical note

Keywords

UN SDGs

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