Sampling and processing blood samples within the South American Youth/Child cARdiovascular and Environmental (SAYCARE) Study

Graciela López, Raphael Assali Serruya, Magalí Barchuk, Diego Gaitan-Charry, Francisco Leonardo Torres-Leal, Luis Alberto Moreno, Carlos Alberto Delgado, Heráclito Barbosa Carvalho, Augusto César Ferreira De Moraes, Gabriela Berg

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2 Scopus citations


Obesity and overweight in children and adolescents is increasing rapidly worldwide; however, scarce data have been reported from South America countries. With the purpose of assessing hyperlipidemia, insulin resistance and chronic inflammation, the evaluation of blood biomarkers such as glucose, lipoproteins and chronic inflammation proteins is required. In the context of the SAYCARE study, in children and adolescents (3 to 18 years) from seven South American cities, our aim was to assess the impact of pre analytical conditions on different biomarkers evaluated in 474 fresh serum samples, in different country centers. We also evaluated the stability according to time and frozen storage within this study across the concordance of the results obtained from the 49 blood samples measured in three different centers. Significant correlations as well as concordance were observed in TG, Total-C, HDL-C and glucose between Buenos Aires and São Paulo. The samples evaluated in Teresina and São Paulo presented similar results, with exception of total cholesterol. We observed acceptable concordance between Buenos Aires vs São Paulo and Teresina vs São Paulo, suggesting that samples could be processed in each of these centers. This concordance is a consequence of the strict pre analytical conditions previously established in the SAYCARE study.

Original languageEnglish
Article number637
JournalScientific Reports
Issue number1
StatePublished - 1 Dec 2020
Externally publishedYes

Bibliographical note

Funding Information:
The SAYCARE study was supported mainly by the Brazilian government from the National Counsel of Technological and Scientific Development (CNPq; proc. 471266/2013-2) and the São Paulo state government from the São Paulo Research Foundation (FAPESP; proc. 2014/11468-6). The SAYCARE study has also been co-funded by other agencies in the other countries: (i) Collaborative Projects Fund (R.D. N°501-2015-INSN-DG-OEA) granted by the Instituto Nacional de Salud del Niño, Lima, Perú; (ii) Sustainability Strategy at the University of Antioquia 2014–2015, Research group of social and economic determinants of health and nutrition, and Demography and Health Research Group at the University of Antioquia, Medellin, Colombia, and Interuniversity Services Corporation (CIS) from UdeA; (iii) Secretary of University Extension and Student Welfare, University of Buenos Aires, Buenos Aires, Argentina; (iv) European Regional Development Fund (MICINN-FEDER) to the GENUD Research Group. The General Coordination of SAYCARE received several scholarships to develop the project: M.D. Raphael A. Serruya was given a Scientific Initiation scholarship from Sao Paulo Research Foundation (proc. 2015/18876–5). Dr. Augusto César F. De Moraes was given a Young Investigator Grant and scholarship from São Paulo Research Foundation — FAPESP (proc. 2017/20317–0 and 2019/02617-1). Full Prof. Luis A. Moreno was given a visiting professor scholarship from the São Paulo Research Foundation—FAPESP (proc. 2015/11406-3). Heráclito B Carvalho received an advanced scientist scholarship from the National Counsel of Technological and Scientific Development (CNPq: proc. 300951/2015-9).


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