Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay

Pedro L. Moro; Hector H. Garcia; Armando E. Gonzales; Juan J. Bonilla; Manuela Verastegui; Robert H. GilmanMD

doi:10.1007/s00436-005-1350-6

Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay

Pedro L. Moro, Hector H. Garcia, Armando E. Gonzales, Juan J. Bonilla, Manuela Verastegui, Robert H. GilmanMD

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Cystic echinococcosis (CE) caused by the larval form of Echinococcus granulosus is a major public health problem in sheep-raising regions of the World. This study compared portable ultrasound with the enzyme-linked immunoelectrotransfer blot (EITB) assay as screening methods to estimate the prevalence of human CE in a remote village in the Peruvian Andes. Three hundred eighty-nine villagers were examined by portable ultrasound and blood samples were drawn by venipuncture. Sera were collected and tested for antibodies against CE using an EITB assay. Cystic lesions were classified based on their ultrasound morphologic characteristics. The prevalence of human CE using portable ultrasound and the EITB assay were 4.9% and 2.6%, respectively. Fifty-three percent of subjects with CE were EITB positive. Portable ultrasound was well received by the community, augmented CE detection and allowed a faster estimate of human infection than the EITB assay.

Original language	English
Pages (from-to)	242-246
Number of pages	5
Journal	Parasitology Research
Volume	96
Issue number	4
DOIs	https://doi.org/10.1007/s00436-005-1350-6
State	Published - Jul 2005
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements Financial support for this study was received from Consejo Nacional de Ciencia y Tecnología (CONCYTEC), Lima, Peru. The methods used in the present study comply with current Peruvian law governing the participation of human subjects in biomedical research.

Keywords

Cystic echinococcosis
Enzyme-linked immunoelectrotransfer blot
Epidemiology
Peru
Ultrasonography

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title = "Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay",

abstract = "Cystic echinococcosis (CE) caused by the larval form of Echinococcus granulosus is a major public health problem in sheep-raising regions of the World. This study compared portable ultrasound with the enzyme-linked immunoelectrotransfer blot (EITB) assay as screening methods to estimate the prevalence of human CE in a remote village in the Peruvian Andes. Three hundred eighty-nine villagers were examined by portable ultrasound and blood samples were drawn by venipuncture. Sera were collected and tested for antibodies against CE using an EITB assay. Cystic lesions were classified based on their ultrasound morphologic characteristics. The prevalence of human CE using portable ultrasound and the EITB assay were 4.9% and 2.6%, respectively. Fifty-three percent of subjects with CE were EITB positive. Portable ultrasound was well received by the community, augmented CE detection and allowed a faster estimate of human infection than the EITB assay.",

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note = "Funding Information: Acknowledgements Financial support for this study was received from Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONCYTEC), Lima, Peru. The methods used in the present study comply with current Peruvian law governing the participation of human subjects in biomedical research.",

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AU - Bonilla, Juan J.

AU - Verastegui, Manuela

AU - GilmanMD, Robert H.

N1 - Funding Information: Acknowledgements Financial support for this study was received from Consejo Nacional de Ciencia y Tecnología (CONCYTEC), Lima, Peru. The methods used in the present study comply with current Peruvian law governing the participation of human subjects in biomedical research.

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N2 - Cystic echinococcosis (CE) caused by the larval form of Echinococcus granulosus is a major public health problem in sheep-raising regions of the World. This study compared portable ultrasound with the enzyme-linked immunoelectrotransfer blot (EITB) assay as screening methods to estimate the prevalence of human CE in a remote village in the Peruvian Andes. Three hundred eighty-nine villagers were examined by portable ultrasound and blood samples were drawn by venipuncture. Sera were collected and tested for antibodies against CE using an EITB assay. Cystic lesions were classified based on their ultrasound morphologic characteristics. The prevalence of human CE using portable ultrasound and the EITB assay were 4.9% and 2.6%, respectively. Fifty-three percent of subjects with CE were EITB positive. Portable ultrasound was well received by the community, augmented CE detection and allowed a faster estimate of human infection than the EITB assay.

AB - Cystic echinococcosis (CE) caused by the larval form of Echinococcus granulosus is a major public health problem in sheep-raising regions of the World. This study compared portable ultrasound with the enzyme-linked immunoelectrotransfer blot (EITB) assay as screening methods to estimate the prevalence of human CE in a remote village in the Peruvian Andes. Three hundred eighty-nine villagers were examined by portable ultrasound and blood samples were drawn by venipuncture. Sera were collected and tested for antibodies against CE using an EITB assay. Cystic lesions were classified based on their ultrasound morphologic characteristics. The prevalence of human CE using portable ultrasound and the EITB assay were 4.9% and 2.6%, respectively. Fifty-three percent of subjects with CE were EITB positive. Portable ultrasound was well received by the community, augmented CE detection and allowed a faster estimate of human infection than the EITB assay.

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Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay

Abstract

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