Short report: Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru

Hirotomo Kato, Abraham G. Cáceres, Eduardo A. Gomez, Tatsuyuki Mimori, Hiroshi Uezato, Jorge D. Marco, Paola A. Barroso, Hiroyuki Iwata, Yoshihisa Hashiguchi

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Sand flies from the Andean areas of Ecuador and Peru were examined for Leishmania infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were Lutzomyia (Lu.) ayacuchensis and Lu. peruensis, respectively. Furthermore, cytochrome b and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as Leishmania (Leishmania) mexicana and L. (Viannia) peruviana, respectively. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.
Original languageAmerican English
Pages (from-to)719-721
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
StatePublished - 1 Nov 2008

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