TY - JOUR
T1 - Short report
T2 - Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru
AU - Kato, Hirotomo
AU - Cáceres, Abraham G.
AU - Gomez, Eduardo A.
AU - Mimori, Tatsuyuki
AU - Uezato, Hiroshi
AU - Marco, Jorge D.
AU - Barroso, Paola A.
AU - Iwata, Hiroyuki
AU - Hashiguchi, Yoshihisa
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2008/11
Y1 - 2008/11
N2 - Sand flies from the Andean areas of Ecuador and Peru were examined for Leishmania infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were Lutzomyia (Lu.) ayacuchensis and Lu. peruensis, respectively. Furthermore, cytochrome b and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as Leishmania (Leishmania) mexicana and L. (Viannia) peruviana, respectively. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research.
AB - Sand flies from the Andean areas of Ecuador and Peru were examined for Leishmania infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were Lutzomyia (Lu.) ayacuchensis and Lu. peruensis, respectively. Furthermore, cytochrome b and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as Leishmania (Leishmania) mexicana and L. (Viannia) peruviana, respectively. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research.
UR - http://www.scopus.com/inward/record.url?scp=55849089250&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.2008.79.719
DO - 10.4269/ajtmh.2008.79.719
M3 - Artículo
C2 - 18981511
AN - SCOPUS:55849089250
SN - 0002-9637
VL - 79
SP - 719
EP - 721
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 5
ER -
