Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru

Germán De la Cruz; Tomás Yuret Miranda; Raúl Humberto Blas; Edgar Neyra; Gisella Orjeda

doi:10.1007/s12230-020-09763-7

Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru

Germán De la Cruz, Tomás Yuret Miranda, Raúl Humberto Blas, Edgar Neyra, Gisella Orjeda

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The Andean native potato is an important food security crop and constitutes a gene pool for potato-breeding. To establish the current state of the native potatoes diversity in the influence zone of the Camisea Gas Project, North Ayacucho, Peru, landraces (n = 144) were collected. Three sampling populations (Anco, Chungui and Ticllas) were resolved using 10 simple sequence repeats (SSRs) markers that amplified 67 polymorphic alleles. Principal component, correspondence and cluster analysis revealed a minimum set of six SSRs to achieve DNA fingerprinting and cost-effective genetic diversity analysis. Analysis of molecular variance (AMOVA), average fixation index (F_ST = 0.279), and genetic distance index (S_B) indicated that the genetic diversity of the native potatoes is high at the intra- and inter-population levels, and each of the three sampling populations constituted closed populations. The three populations were genetically distinct and contained unique genotypes and exclusive alleles. Misidentified native potatoes (10.41%) were found.

Original language	English
Pages (from-to)	143-161
Number of pages	19
Journal	American Journal of Potato Research
Volume	97
Issue number	2
DOIs	https://doi.org/10.1007/s12230-020-09763-7
State	Published - 1 Apr 2020

Bibliographical note

Funding Information:
The authors wish to thank Dr. William Roca Pizzini (International Potato Center (CIP), Peru) for his review and analysis of the first draft of this manuscript. The authors too would like to thank Enago ( www.enago.com ) for the English language review. This work was supported by the Socioeconomic Development Fund of Camisea (ley N° 28451-MEF-PERU) of the Universidad Nacional San Cristóbal de Huamanga, Ayacucho, Peru. The work was carried out through inter-institutional collaboration with Universidad Peruana Cayetano Heredia (UPCH).

Funding Information:
The authors wish to thank Dr. William Roca Pizzini (International Potato Center (CIP), Peru) for his review and analysis of the first draft of this manuscript. The authors too would like to thank Enago (www.enago.com) for the English language review. This work was supported by the Socioeconomic Development Fund of Camisea (ley N? 28451-MEF-PERU) of the Universidad Nacional San Crist?bal de Huamanga, Ayacucho, Peru. The work was carried out through inter-institutional collaboration with Universidad Peruana Cayetano Heredia (UPCH).

Publisher Copyright:
© 2020, The Potato Association of America.

Keywords

AMOVA
Allele
F
Landrace
Microsatellite
Polymorphism

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10.1007/s12230-020-09763-7

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title = "Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru",

abstract = "The Andean native potato is an important food security crop and constitutes a gene pool for potato-breeding. To establish the current state of the native potatoes diversity in the influence zone of the Camisea Gas Project, North Ayacucho, Peru, landraces (n = 144) were collected. Three sampling populations (Anco, Chungui and Ticllas) were resolved using 10 simple sequence repeats (SSRs) markers that amplified 67 polymorphic alleles. Principal component, correspondence and cluster analysis revealed a minimum set of six SSRs to achieve DNA fingerprinting and cost-effective genetic diversity analysis. Analysis of molecular variance (AMOVA), average fixation index (FST = 0.279), and genetic distance index (SB) indicated that the genetic diversity of the native potatoes is high at the intra- and inter-population levels, and each of the three sampling populations constituted closed populations. The three populations were genetically distinct and contained unique genotypes and exclusive alleles. Misidentified native potatoes (10.41%) were found.",

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Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru. / De la Cruz, Germán; Miranda, Tomás Yuret; Blas, Raúl Humberto et al.
In: American Journal of Potato Research, Vol. 97, No. 2, 01.04.2020, p. 143-161.

Research output: Contribution to journal › Article › peer-review

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AU - Blas, Raúl Humberto

AU - Neyra, Edgar

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AB - The Andean native potato is an important food security crop and constitutes a gene pool for potato-breeding. To establish the current state of the native potatoes diversity in the influence zone of the Camisea Gas Project, North Ayacucho, Peru, landraces (n = 144) were collected. Three sampling populations (Anco, Chungui and Ticllas) were resolved using 10 simple sequence repeats (SSRs) markers that amplified 67 polymorphic alleles. Principal component, correspondence and cluster analysis revealed a minimum set of six SSRs to achieve DNA fingerprinting and cost-effective genetic diversity analysis. Analysis of molecular variance (AMOVA), average fixation index (FST = 0.279), and genetic distance index (SB) indicated that the genetic diversity of the native potatoes is high at the intra- and inter-population levels, and each of the three sampling populations constituted closed populations. The three populations were genetically distinct and contained unique genotypes and exclusive alleles. Misidentified native potatoes (10.41%) were found.

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Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru

Abstract

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Keywords

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