Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry

Pierina Sueli Bonato; Anderson Rodrigo Moraes de Oliveira; Fernando José Malagueño de Santana; Bruno José Dumet Fernandes; Vera Lucia Lanchote; Armando E. Gonzalez; Hector H. Garcia; Osvaldo Massaiti Takayanagui

doi:10.1016/j.jpba.2006.11.020

Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry

Pierina Sueli Bonato, Anderson Rodrigo Moraes de Oliveira, Fernando José Malagueño de Santana, Bruno José Dumet Fernandes, Vera Lucia Lanchote, Armando E. Gonzalez, Hector H. Garcia, Osvaldo Massaiti Takayanagui

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.

Original language	English
Pages (from-to)	558-563
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/j.jpba.2006.11.020
State	Published - 28 Jun 2007
Externally published	Yes

Bibliographical note

Funding Information:
The authors are grateful to Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the National Institutes of Health, USA (Grant NI-51976) for financial support and for granting research fellowship.

Keywords

Albendazole sulfone
Albendazole sulfoxide
LC-MS-MS
Plasma
Praziquantel
trans-4-Hydroxypraziquantel

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10.1016/j.jpba.2006.11.020

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Bonato, P. S., Oliveira, A. R. M. D., Santana, F. J. M. D., Fernandes, B. J. D., Lanchote, V. L., Gonzalez, A. E., Garcia, H. H., & Takayanagui, O. M. (2007). Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 44(2), 558-563. https://doi.org/10.1016/j.jpba.2006.11.020

Bonato, Pierina Sueli ; Oliveira, Anderson Rodrigo Moraes de ; Santana, Fernando José Malagueño de et al. / Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry. In: Journal of Pharmaceutical and Biomedical Analysis. 2007 ; Vol. 44, No. 2. pp. 558-563.

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title = "Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry",

abstract = "The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.",

keywords = "Albendazole sulfone, Albendazole sulfoxide, LC-MS-MS, Plasma, Praziquantel, trans-4-Hydroxypraziquantel",

author = "Bonato, {Pierina Sueli} and Oliveira, {Anderson Rodrigo Moraes de} and Santana, {Fernando Jos{\'e} Malague{\~n}o de} and Fernandes, {Bruno Jos{\'e} Dumet} and Lanchote, {Vera Lucia} and Gonzalez, {Armando E.} and Garcia, {Hector H.} and Takayanagui, {Osvaldo Massaiti}",

note = "Funding Information: The authors are grateful to Funda{\c c}{\~a}o de Amparo a Pesquisa do Estado de S{\~a}o Paulo (FAPESP), Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES) and Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) and the National Institutes of Health, USA (Grant NI-51976) for financial support and for granting research fellowship.",

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Bonato, PS, Oliveira, ARMD, Santana, FJMD, Fernandes, BJD, Lanchote, VL, Gonzalez, AE, Garcia, HH & Takayanagui, OM 2007, 'Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry', Journal of Pharmaceutical and Biomedical Analysis, vol. 44, no. 2, pp. 558-563. https://doi.org/10.1016/j.jpba.2006.11.020

Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry. / Bonato, Pierina Sueli; Oliveira, Anderson Rodrigo Moraes de; Santana, Fernando José Malagueño de et al.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 44, No. 2, 28.06.2007, p. 558-563.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry

AU - Bonato, Pierina Sueli

AU - Oliveira, Anderson Rodrigo Moraes de

AU - Santana, Fernando José Malagueño de

AU - Fernandes, Bruno José Dumet

AU - Lanchote, Vera Lucia

AU - Gonzalez, Armando E.

AU - Garcia, Hector H.

AU - Takayanagui, Osvaldo Massaiti

N1 - Funding Information: The authors are grateful to Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the National Institutes of Health, USA (Grant NI-51976) for financial support and for granting research fellowship.

PY - 2007/6/28

Y1 - 2007/6/28

N2 - The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.

AB - The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.

KW - Albendazole sulfone

KW - Albendazole sulfoxide

KW - LC-MS-MS

KW - Plasma

KW - Praziquantel

KW - trans-4-Hydroxypraziquantel

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U2 - 10.1016/j.jpba.2006.11.020

DO - 10.1016/j.jpba.2006.11.020

M3 - Artículo

C2 - 17169522

AN - SCOPUS:34249940916

SN - 0731-7085

VL - 44

SP - 558

EP - 563

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 2

ER -

Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry

Abstract

Bibliographical note

Keywords

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