Staining of antinuclear antibodies and antibodies against removable nuclear antigens in connective tissue diseases

José Enrique Oliva Menacho, Jorge Luis Arroyo Acevedo, José Arturo Oliva-Candela, Marco Antonio García-Hjarles, Lester Domínguez-Huarcaya

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction and objectives: Connective tissue diseases are inflammatory, autoimmune diseases and threaten quality of life. To determine the relationship between staining patterns of antinuclear antibodies and antibodies against extractable nuclear antigens in patients with connective tissue disease. Materials and methods: Observational, basic, analytical and transversal study. Study conducted in the Immunology Service of the Arzobispo Loayza National Hospital between January 2017 and June 2017. We analyzed 291 samples of patients with CTD and for the detection of anti-nuclear antibody staining patterns, the immunological kit and observation with microscope of at 40X Immunofluorescence and for the detection of the antibodies against extractable nuclear antigens. The Immunoblot method was employed. Statistical analyses were carried out with the statistical package SPSS version 21 for Windows. We used the Pearson Chi-square test for the categorical variables, a value of p < 0.05 was considered significant. Results: There was a significant relationship p < 0.05 of the homogeneous pattern, the mottled pattern with Anti-histones (p = 0.000), Anti-nucleosomes (p = 0.000), Anti-Ro 52 (p = 0.000), Anti-SSA (p = 0.001), Anti-SSB (p = 0.003), Anti-dsDNA (p = 0.000) with the Pearson Chi-square test. There was a significant relationship of p < 0.05 of the centromeric pattern with Anti-Cenp B (p = 0.000) with Fisher⿿s exact statistic. Conclusions: There was a significant relationship between the anti-nuclear antibody staining patterns and the antibodies to the core extractable antigens in patients with systemic lupus erythematosus, Sjögren⿿s syndrome, Calcinosis, Raynaud⿿s phenomenon, esophageal Dysmotility, sclerodactyly and Telangiectasia (CREST), Scleroderma and Polymyositis.

Original languageEnglish
Pages (from-to)18-25
Number of pages8
JournalAllergologia et Immunopathologia
Volume48
Issue number1
DOIs
StatePublished - 1 Jan 2020
Externally publishedYes

Bibliographical note

Funding Information:
An observational, analytical and cross-sectional study was conducted at the Immunology Service of the Arzobispo Loayza National Hospital between January and June 2017. A total of 291 clinical histories were reviewed and blood samples from patients with connective tissue disease were processed. For the detection of staining patterns of antinuclear antibodies in the patients⿿ sera, the immunological kit and observation with a 40x immunofluorescence microscope were used. For the detection of antibodies against extractable nuclear antigens in patients⿿ sera, the Immunoblot method was used. This study was conducted in accordance with the ethical principles that have their origin in the Declaration of Helsinki, was evaluated and approved by the research ethics committee of the Institute of Ethics in Health of the National University of San Marcos Act No. 1811 with Project Code No. 0014; the research work was approved by the Institutional Research Committee and the General Directorate of the Arzobispo Loayza National Hospital with File N ° 007645.

Keywords

  • Anti-Nuclear antibody staining pattern
  • Antibodies against extractable nuclear antigens
  • Connective tissue diseases
  • Immunoblot
  • Indirect immunofluorescence

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