Standardization and validation of qualitative realtime RT-PCR for detection of classical swine fever virus

Keyphrases

• Real-time RT-PCR 100%
• Classical Swine Fever Virus 100%
• Real-time RT-PCR Assay 60%
• Negative Control 30%
• Immunofluorescence Assay 30%
• Positive Control 30%
• Virus Isolation 20%
• Brescia 20%
• 5′-untranslated Region (5′-UTR) 20%
• Complementary DNA (cDNA) 10%
• High Sensitivity 10%
• Conserved Regions 10%
• Field Sampling 10%
• Positive Predictive Value 10%
• High Specificity 10%
• Commercial Kits 10%
• Bovine Viral Diarrhea Virus 10%
• Tonsil 10%
• DNA Synthesis 10%
• Lymph 10%
• Cycle Threshold 10%
• Porcine Rotavirus 10%
• E2 Glycoprotein 10%
• Reference Strain 10%
• Negative Samples 10%
• Border Disease Virus 10%
• Nodule 10%
• Viral RNA Extraction 10%
• Negative Value 10%

Immunology and Microbiology

• Quantitative Reverse Transcription Polymerase Chain Reaction 100%
• Classical Swine Fever Virus 100%
• Immunofluorescence 30%
• 5' Untranslated Region 20%
• Virus Isolation 20%
• Vaccine Efficacy 10%
• Lymph Node 10%
• Glycoprotein 10%
• Tonsil 10%
• RNA Extraction 10%
• Bovine Viral Diarrhea Virus 10%
• Melting Temperature 10%
• Cycle Threshold 10%
• Border Disease Virus 10%
• Porcine Rotavirus 10%
• DNA Synthesis 10%

Veterinary Science and Veterinary Medicine

• Classical Swine Fever 100%
• Bovine Virus Diarrhea 10%