Técnicas de aislamiento y purificación de ooquistes de Sarcocystis aucheniae a partir de intestino delgado de perros experimentalmente infectados

Translated title of the contribution: Techniques for the isolation and purification of Sarcocystis aucheniae oocysts from small intestine of experimentally infected dogs

S. Fiorella Zacarías, T. Rosa Sam, D. Daphne Ramos, Orlando Alberto Lucas Aguirre, L. Juan Lucas

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Procedures were evaluated for the rapid and efficient purification of oocysts and esporocysts of Sarcocystis aucheniae (Sa) from the small intestine of experimentally infected dogs with 400 Sa macrocysts obtained from meat of naturally infected alpacas. The mucosa of the small intestine was removed. Samples were washed by centrifugation with PBS, homogenized and centrifuged. Large quantities of oocysts attached to the intestinal microvilli were obtained. In the first procedure, an ezimatic treatment with 5% tripsin was used resulting in 50% oocyst separation. In the second procedure samples were treated with 2.6% sodium hypoclorite solution achieving 80% separation. In the third procedure, samples were centrifuged with a discontinuous density gradient of potassium bromide rendering 99% oocyst separation. The later procedure allowed obtaining relevant quantities of purified Sa oocysts and without loss of viability.

Translated title of the contributionTechniques for the isolation and purification of Sarcocystis aucheniae oocysts from small intestine of experimentally infected dogs
Original languageSpanish
Pages (from-to)396-403
Number of pages8
JournalRevista de Investigaciones Veterinarias del Peru
Volume24
Issue number3
StatePublished - Aug 2013
Externally publishedYes

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