The heritability and patterns of DNA methylation in normal human colorectum

Amy Rowlatt, Gustavo Hernández-Suárez, María Carolina Sanabria-Salas, Martha Serrano-López, Konrad Rawlik, Eva Hernandez-Illan, Cristina Alenda, Adela Castillejo, Jose Luis Soto, Chris S. Haley, Albert Tenesa

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

DNA methylation (DNAm) has been linked to changes in chromatin structure, gene expression and disease. The DNAm level can be affected by genetic variation; although, how this differs by CpG dinucleotide density and genic location of the DNAm site is not well understood. Moreover, the effect of disease causing variants on the DNAm level in a tissue relevant to disease has yet to be fully elucidated. To this end, we investigated the phenotypic profiles, genetic effects and regional genomic heritability for 196080 DNAm sites in healthy colorectum tissue from 132 unrelated Colombian individuals. DNAm sites in regions of low-CpG density were more variable, on average more methylated and were more likely to be significantly heritable when compared with DNAm sites in regions of high-CpG density. DNAm sites located in intergenic regions had a higher mean DNAm level and were more likely to be heritable when compared with DNAm sites in the transcription start site (TSS) of a gene expressed in colon tissue. Within CpG-dense regions, the propensity of the DNAm level to be heritable was lower in the TSS of genes expressed in colon tissue than in the TSS of genes not expressed in colon tissue. In addition, regional genetic variation was associated with variation in local DNAm level no more frequently for DNAm sites within colorectal cancer risk regions than it was for DNAm sites outside such regions. Overall, DNAm sites located in different genomic contexts exhibited distinguishable profiles and may have a different biological function.

Original languageEnglish
Pages (from-to)2600-2611
Number of pages12
JournalHuman Molecular Genetics
Volume25
Issue number12
DOIs
StatePublished - 15 Jun 2016

Bibliographical note

Publisher Copyright:
© The Author 2016. Published by Oxford University Press.

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