The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça, Camila S. Palma, Daniele Albuquerque, Gabriela Norma Solano Canchaya, Mariana L. Grassi, Vera L. Epifânio, Eduardo B. de Oliveira

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

Original languageEnglish
Pages (from-to)323-335
Number of pages13
JournalMethods in Molecular Biology
Volume1156
DOIs
StatePublished - 2014

Keywords

  • Cancer
  • Mass spectrometry
  • Multiple reaction monitoring
  • Protein fractionation
  • SILAC
  • Secretome

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