The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça; Camila S. Palma; Daniele Albuquerque; Gabriela N.S. Canchaya; Mariana L. Grassi; Vera L. Epifânio; Eduardo B. de Oliveira

doi:10.1007/978-1-4939-0685-7_22

The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça, Camila S. Palma, Daniele Albuquerque, Gabriela N.S. Canchaya, Mariana L. Grassi, Vera L. Epifânio, Eduardo B. de Oliveira

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

Original language	English
Pages (from-to)	323-335
Number of pages	13
Journal	Methods in Molecular Biology
Volume	1156
DOIs	https://doi.org/10.1007/978-1-4939-0685-7_22
State	Published - 2014

Bibliographical note

Publisher Copyright:
© 2014, Springer Science+Business Media New York.

Keywords

Cancer
Mass spectrometry
Multiple reaction monitoring
Protein fractionation
SILAC
Secretome

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10.1007/978-1-4939-0685-7_22

Cite this

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abstract = "The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.",

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AU - Faça, Vitor M.

AU - Palma, Camila S.

AU - Albuquerque, Daniele

AU - Canchaya, Gabriela N.S.

AU - Grassi, Mariana L.

AU - Epifânio, Vera L.

AU - de Oliveira, Eduardo B.

PY - 2014

Y1 - 2014

N2 - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

AB - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

KW - Cancer

KW - Mass spectrometry

KW - Multiple reaction monitoring

KW - Protein fractionation

KW - SILAC

KW - Secretome

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DO - 10.1007/978-1-4939-0685-7_22

M3 - Artículo

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AN - SCOPUS:84922393306

SN - 1064-3745

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Abstract

Bibliographical note

Keywords

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