Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry

Hannah E. Steinberg; Paul Russo; Noelia Angulo; Raúl Ynocente; Cristina Montoya; Andrea Diestra; Cusi Ferradas; Francesca Schiaffino; Edgar Florentini; Juan Jimenez; Maritza Calderón; Vern B. Carruthers; Robert H. Gilman; Lance Liotta; Alessandra Luchini

doi:10.1016/j.nano.2017.11.020

Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry

Hannah E. Steinberg, Paul Russo, Noelia Angulo, Raúl Ynocente, Cristina Montoya, Andrea Diestra, Cusi Ferradas, Francesca Schiaffino, Edgar Florentini, Juan Jimenez, Maritza Calderón, Vern B. Carruthers, Robert H. Gilman, Lance Liotta, Alessandra Luchini

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven Toxoplasma gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.

Original language	English
Pages (from-to)	461-469
Number of pages	9
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/j.nano.2017.11.020
State	Published - Feb 2018

Bibliographical note

Funding Information:
Funding from INNOVATE PERU (137-PNICP-PIAP) allowed for the collaboration of Universidad Peruana Cayetano Heredia and Universidad Nacional Mayor de San Marcos as well as equipment, training and materials. Dr. Gilman's 1D43TW010074-01 supported the training of many of the Peruvian authors. This study was partially funded by the following grants to Dr. Liotta: National Institutes of Health NCI R33CA173359 and R33CA206937, NIAID R21AI099851 and R21AI117425, and NIAMS 1R01AR068436. Drs. Liotta and Luchini are inventors on patents related to the nanoparticles. Ceres Nanosciences licensed the rights of these patents that are owned by George Mason University. Drs. Liotta and Luchini own shares of Ceres Nanosciences.

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

Multiple reactions monitoring
Nanoparticles
Toxoplasma gondii

Access to Document

10.1016/j.nano.2017.11.020

Cite this

Steinberg, H. E., Russo, P., Angulo, N., Ynocente, R., Montoya, C., Diestra, A., Ferradas, C., Schiaffino, F., Florentini, E., Jimenez, J., Calderón, M., Carruthers, V. B., Gilman, R. H., Liotta, L., & Luchini, A. (2018). Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry. Nanomedicine: Nanotechnology, Biology, and Medicine, 14(2), 461-469. https://doi.org/10.1016/j.nano.2017.11.020

@article{4d90d7d4f43f4f8b9e0cc299e66e007f,

title = "Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry",

abstract = "Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven Toxoplasma gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.",

keywords = "Multiple reactions monitoring, Nanoparticles, Toxoplasma gondii",

author = "Steinberg, {Hannah E.} and Paul Russo and Noelia Angulo and Ra{\'u}l Ynocente and Cristina Montoya and Andrea Diestra and Cusi Ferradas and Francesca Schiaffino and Edgar Florentini and Juan Jimenez and Maritza Calder{\'o}n and Carruthers, {Vern B.} and Gilman, {Robert H.} and Lance Liotta and Alessandra Luchini",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2018",

month = feb,

doi = "10.1016/j.nano.2017.11.020",

language = "Ingl{\'e}s",

volume = "14",

pages = "461--469",

journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

issn = "1549-9634",

publisher = "Elsevier Inc.",

number = "2",

}

Steinberg, HE, Russo, P, Angulo, N, Ynocente, R, Montoya, C, Diestra, A, Ferradas, C, Schiaffino, F, Florentini, E, Jimenez, J, Calderón, M, Carruthers, VB, Gilman, RH, Liotta, L & Luchini, A 2018, 'Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 14, no. 2, pp. 461-469. https://doi.org/10.1016/j.nano.2017.11.020

TY - JOUR

T1 - Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry

AU - Steinberg, Hannah E.

AU - Russo, Paul

AU - Angulo, Noelia

AU - Ynocente, Raúl

AU - Montoya, Cristina

AU - Diestra, Andrea

AU - Ferradas, Cusi

AU - Schiaffino, Francesca

AU - Florentini, Edgar

AU - Jimenez, Juan

AU - Calderón, Maritza

AU - Carruthers, Vern B.

AU - Gilman, Robert H.

AU - Liotta, Lance

AU - Luchini, Alessandra

PY - 2018/2

Y1 - 2018/2

N2 - Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven Toxoplasma gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.

AB - Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven Toxoplasma gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.

KW - Multiple reactions monitoring

KW - Nanoparticles

KW - Toxoplasma gondii

UR - http://www.scopus.com/inward/record.url?scp=85039736424&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2017.11.020

DO - 10.1016/j.nano.2017.11.020

M3 - Artículo

C2 - 29203146

AN - SCOPUS:85039736424

SN - 1549-9634

VL - 14

SP - 461

EP - 469

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

IS - 2

ER -

Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this