Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells

Victor Izaguirre, José M. Fernández-Fernández, Valentín Ceña, Carmen González-García

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Nicotine-induced ATP secretion from chromaffin cells was blocked by imipramine and desipramine. This blocking action took place on both, fast and slow, components of ATP secretion. Exposure of chromaffin cells to nicotine (10 μM) for 4 s induced an inward current of about -155 pA. Imipramine and desipramine blocked, in a concentration-dependent manner, both peak inward current and total charge influx in response to nicotine. In addition, imipramine and desipramine partially (40%) blocked depolarization-induced ATP secretion and Ca2+ currents evoked by high K+. This suggests that tricyclic antidepressants block nicotine-induced ATP secretion by acting on two targets: one is the nicotinic receptor itself and the second one are voltage-dependent Ca2+ channels.

Original languageEnglish
Pages (from-to)39-42
Number of pages4
JournalFEBS Letters
Volume418
Issue number1-2
DOIs
StatePublished - 24 Nov 1997

Bibliographical note

Funding Information:
The authors thank Mrs. Esperanza Gascó for superb technical assistance. This work has been supported in part by grants from Fondo de Investigaciones Sanitarias (95/1655) to C.G.G.; from DGICYT (PM92-0112), Comisión Interministerial de Ciencia y Tecnologı́a, Plan Nacional de Salud (SAF96-0169) and Fundación Navarro-Trı́podi to V.C.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

  • ATP secretion
  • Calcium channel
  • Chromaffin cell
  • Desipramine
  • Imipramine
  • Nicotine

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