Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells

Victor Izaguirre; José M. Fernández-Fernández; Valentín Ceña; Carmen González-García

doi:10.1016/S0014-5793(97)01343-4

Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells

Victor Izaguirre, José M. Fernández-Fernández, Valentín Ceña, Carmen González-García

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Nicotine-induced ATP secretion from chromaffin cells was blocked by imipramine and desipramine. This blocking action took place on both, fast and slow, components of ATP secretion. Exposure of chromaffin cells to nicotine (10 μM) for 4 s induced an inward current of about -155 pA. Imipramine and desipramine blocked, in a concentration-dependent manner, both peak inward current and total charge influx in response to nicotine. In addition, imipramine and desipramine partially (40%) blocked depolarization-induced ATP secretion and Ca²⁺ currents evoked by high K⁺. This suggests that tricyclic antidepressants block nicotine-induced ATP secretion by acting on two targets: one is the nicotinic receptor itself and the second one are voltage-dependent Ca²⁺ channels.

Original language	English
Pages (from-to)	39-42
Number of pages	4
Journal	FEBS Letters
Volume	418
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(97)01343-4
State	Published - 24 Nov 1997

Bibliographical note

Funding Information:
The authors thank Mrs. Esperanza Gascó for superb technical assistance. This work has been supported in part by grants from Fondo de Investigaciones Sanitarias (95/1655) to C.G.G.; from DGICYT (PM92-0112), Comisión Interministerial de Ciencia y Tecnologı́a, Plan Nacional de Salud (SAF96-0169) and Fundación Navarro-Trı́podi to V.C.

Keywords

ATP secretion
Calcium channel
Chromaffin cell
Desipramine
Imipramine
Nicotine

Access to Document

10.1016/S0014-5793(97)01343-4

Cite this

@article{fcd8fe33f05b49ac9c9575b7e5475e4b,

title = "Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells",

abstract = "Nicotine-induced ATP secretion from chromaffin cells was blocked by imipramine and desipramine. This blocking action took place on both, fast and slow, components of ATP secretion. Exposure of chromaffin cells to nicotine (10 μM) for 4 s induced an inward current of about -155 pA. Imipramine and desipramine blocked, in a concentration-dependent manner, both peak inward current and total charge influx in response to nicotine. In addition, imipramine and desipramine partially (40%) blocked depolarization-induced ATP secretion and Ca2+ currents evoked by high K+. This suggests that tricyclic antidepressants block nicotine-induced ATP secretion by acting on two targets: one is the nicotinic receptor itself and the second one are voltage-dependent Ca2+ channels.",

keywords = "ATP secretion, Calcium channel, Chromaffin cell, Desipramine, Imipramine, Nicotine",

author = "Victor Izaguirre and Fern{\'a}ndez-Fern{\'a}ndez, {Jos{\'e} M.} and Valent{\'i}n Ce{\~n}a and Carmen Gonz{\'a}lez-Garc{\'i}a",

note = "Funding Information: The authors thank Mrs. Esperanza Gasc{\'o} for superb technical assistance. This work has been supported in part by grants from Fondo de Investigaciones Sanitarias (95/1655) to C.G.G.; from DGICYT (PM92-0112), Comisi{\'o}n Interministerial de Ciencia y Tecnolog{\'ı}a, Plan Nacional de Salud (SAF96-0169) and Fundaci{\'o}n Navarro-Tr{\'ı}podi to V.C.",

year = "1997",

month = nov,

day = "24",

doi = "10.1016/S0014-5793(97)01343-4",

language = "Ingl{\'e}s",

volume = "418",

pages = "39--42",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1-2",

}

TY - JOUR

T1 - Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells

AU - Izaguirre, Victor

AU - Fernández-Fernández, José M.

AU - Ceña, Valentín

AU - González-García, Carmen

N1 - Funding Information: The authors thank Mrs. Esperanza Gascó for superb technical assistance. This work has been supported in part by grants from Fondo de Investigaciones Sanitarias (95/1655) to C.G.G.; from DGICYT (PM92-0112), Comisión Interministerial de Ciencia y Tecnologı́a, Plan Nacional de Salud (SAF96-0169) and Fundación Navarro-Trı́podi to V.C.

PY - 1997/11/24

Y1 - 1997/11/24

N2 - Nicotine-induced ATP secretion from chromaffin cells was blocked by imipramine and desipramine. This blocking action took place on both, fast and slow, components of ATP secretion. Exposure of chromaffin cells to nicotine (10 μM) for 4 s induced an inward current of about -155 pA. Imipramine and desipramine blocked, in a concentration-dependent manner, both peak inward current and total charge influx in response to nicotine. In addition, imipramine and desipramine partially (40%) blocked depolarization-induced ATP secretion and Ca2+ currents evoked by high K+. This suggests that tricyclic antidepressants block nicotine-induced ATP secretion by acting on two targets: one is the nicotinic receptor itself and the second one are voltage-dependent Ca2+ channels.

AB - Nicotine-induced ATP secretion from chromaffin cells was blocked by imipramine and desipramine. This blocking action took place on both, fast and slow, components of ATP secretion. Exposure of chromaffin cells to nicotine (10 μM) for 4 s induced an inward current of about -155 pA. Imipramine and desipramine blocked, in a concentration-dependent manner, both peak inward current and total charge influx in response to nicotine. In addition, imipramine and desipramine partially (40%) blocked depolarization-induced ATP secretion and Ca2+ currents evoked by high K+. This suggests that tricyclic antidepressants block nicotine-induced ATP secretion by acting on two targets: one is the nicotinic receptor itself and the second one are voltage-dependent Ca2+ channels.

KW - ATP secretion

KW - Calcium channel

KW - Chromaffin cell

KW - Desipramine

KW - Imipramine

KW - Nicotine

UR - http://www.scopus.com/inward/record.url?scp=0343416367&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(97)01343-4

DO - 10.1016/S0014-5793(97)01343-4

M3 - Artículo

C2 - 9414091

AN - SCOPUS:0343416367

SN - 0014-5793

VL - 418

SP - 39

EP - 42

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this