The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification.
|Number of pages||6|
|Journal||Revista Peruana de Medicina Experimental y Salud Publica|
|State||Published - 2020|
Bibliographical noteFunding Information:
We thank Dr. Cristina Guerra and Dr. Miguel Orrego for facilitating access to hybridoma supernatants. To Adriana Paredes, for her guidance in the initial steps of the study. To Helena Jahuira, and Giuliana Oyola Lozada for coordinating the purification procedures. To Heydi Toro for the administrative and financial coordination of the project. To Doctors Patricia Sheen, Manuela Verastegui, and Jos? Espinoza for providing us with access to their laboratory equipment. To Dr. Theodore Nash Sukwan Handali for corrections and suggestions in writing the article. Funding sources: This study was funded by the National Council for Science, Technology, and Technological Innovation (FONDECYT - CIENCIA ACTIVA) [115-2015-FONDECYT-DE] and the NIH/ Fogarty Training Grant [TW001140].
© 2020, Instituto Nacional de Salud. All rights reserved.
- Monoclonal Antibodies
- Taenia solium