Variations in the protein composition, enzymatic and biological activities in the venom of Bothrops atrox snake (viperidae) in relation to age

O. Málaga, C. Pantigoso, Y. Morante, V. Hercdia, J. Cárdenas, A. Yarlequé

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Abstract

© Facultad de Ciencias Biològicas UNMSM. Bothrops atrox is the main venomous snake in the Amazonic region of Peru, because it is responsible of the majority of human ophidic accidents. For this reason. variation into chemical composition as well as enzymatic activities on this venom were studied in relation to age, considering 3 groups of captivity snakes: young of 1 and 2 years respectively as well as adult, major of 5 years. The venom obtained Irom the three groups was pooled, Iyophilized and properly dissolved into saline solution. Protein concentration at 280 nm and by Lowry's method was calculated into each venom sample. Besides, protein bands were determined by PAGE-SDS. In addition, the following enzymatic acti vities were determined caseinolytic, Amidolytic on BAPNA, coagulant, hialuronidase, L- Aminoacidoxidase (Lao) and fosfolipase A2 . Biological eflects such as hemorrhage and edema were as sayed on white mice. The results showed that high protein concentration (0,938 mg protein/mg venom) and a bigger number of protein bands (8 bands) were found in the venom from young snakes (2 years). Besides, amidolytic, foslolipase A, and Lao as well as hemorrhagic and edemati<: Effects were the most highest in the same samples. In contrast, caseinolytic and coagulant activities were found to increase in proportion to age. Hialuronidase activity decreased with age instead . It was interesting to observe that the venom from adult snakes had only 6,4% folfolipasẽactivity, taking into consideration that the maximum value was obtained with sample of young snakes (2 years).
Original languageAmerican English
Pages (from-to)161-170
Number of pages10
JournalRevista Peruana de Biologia
DOIs
StatePublished - 1 Jan 2000

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