@article{90c561da918e4bc4ad0afc2daee89253,
title = "ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells",
abstract = "We have studied the contribution of N-type voltage-dependent Ca2+ channels to both norepinephrine and epinephrine secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-conotoxin GVIA, a selective blocker of N-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced catecholamine secretion was not affected by exposure of bovine chromaffin cells to ω-conotoxin GVIA (1 μM). However, nicotine-induced both norepinephrine and epinephrine secretion were similarly blocked (about 25%) by ω-conotoxin GVIA (1 μM). This effect could be explained by a potent (about 80%) and reversible blockade of the inward current induced by nicotine receptor activation in bovine chromaffin cells. The results indicate that besides the blockade of N-type voltage-dependent channels, ω-conotoxin GVIA is a potent and reversible blocker of the nicotinic receptor-induced currents in chromaffin cells.",
keywords = "Chromaffin cells, Nicotine-induced catecholamine secretion, Nicotinic receptor-induced currents, ω-Conotoxin",
author = "Fern{\'a}ndez, {Jos{\'e}M M.} and Ricardo Granja and Victor Izaguirre and Carmen Gonz{\'a}lez-Garc{\'i}a and Valent{\'i}n Ce{\~n}a",
note = "Funding Information: 4,8 y---3r---vtinic receptor activation in other neurosecretory systems. -L--This work has been supported, in part, by grants from 15 Fundacion Salud 2000, Direccicin General de Investiga-Fig. 2. Effect of o-conotoxin GVIA on nicotine-induced currents. In- cion Cientifica y Tecnica, grant #PM92-0112 and the (10pM)with an interval of 15 min as described in the text. During theward current induced by three consecutive applications of nicotine European Economic Community, grant #SCl*-CT91-second application of nicotine w-conotoxin GVIA (1 PM) was present, 0709. R.G. is supported by a University of Alicante fel-The figure represent a typical experiment that was repeated four times lowship; J.M.F. by a CAM fellowship and V.I. by a Gen-with similar results. eralitat Valenciana fellowship.",
year = "1995",
month = may,
day = "19",
doi = "10.1016/0304-3940(95)11558-X",
language = "Ingl{\'e}s",
volume = "191",
pages = "59--62",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",
}