ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells

JoséM M. Fernández, Ricardo Granja, Victor Izaguirre, Carmen González-García, Valentín Ceña

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

14 Citas (Scopus)

Resumen

We have studied the contribution of N-type voltage-dependent Ca2+ channels to both norepinephrine and epinephrine secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-conotoxin GVIA, a selective blocker of N-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced catecholamine secretion was not affected by exposure of bovine chromaffin cells to ω-conotoxin GVIA (1 μM). However, nicotine-induced both norepinephrine and epinephrine secretion were similarly blocked (about 25%) by ω-conotoxin GVIA (1 μM). This effect could be explained by a potent (about 80%) and reversible blockade of the inward current induced by nicotine receptor activation in bovine chromaffin cells. The results indicate that besides the blockade of N-type voltage-dependent channels, ω-conotoxin GVIA is a potent and reversible blocker of the nicotinic receptor-induced currents in chromaffin cells.

Idioma originalInglés
Páginas (desde-hasta)59-62
Número de páginas4
PublicaciónNeuroscience Letters
Volumen191
N.º1-2
DOI
EstadoPublicada - 19 may 1995

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