A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

W. H. Roldán; F. Martins De Paula; R. C.B. Gryschek

doi:10.1017/S0022149X20000930

A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

W. H. Roldán, F. Martins De Paula, R. C.B. Gryschek

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Resumen

The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.

Idioma original	Inglés
Número de artículo	e210
Publicación	Journal of Helminthology
DOI	https://doi.org/10.1017/S0022149X20000930
Estado	Aceptada/en prensa - 2020
Publicado de forma externa	Sí

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Copyright © The Author(s), 2020. Published by Cambridge University Press.

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title = "A simple method for purification of Strongyloides venezuelensis eggs from rat faeces",

abstract = "The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.",

keywords = "Strongyloides, egg, faeces, method, nematode, purification",

author = "Rold{\'a}n, {W. H.} and {Martins De Paula}, F. and Gryschek, {R. C.B.}",

note = "Publisher Copyright: Copyright {\textcopyright} The Author(s), 2020. Published by Cambridge University Press.",

year = "2020",

doi = "10.1017/S0022149X20000930",

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journal = "Journal of Helminthology",

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T1 - A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

AU - Roldán, W. H.

AU - Martins De Paula, F.

AU - Gryschek, R. C.B.

PY - 2020

Y1 - 2020

N2 - The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.

AB - The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.

KW - Strongyloides

KW - egg

KW - faeces

KW - method

KW - nematode

KW - purification

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DO - 10.1017/S0022149X20000930

M3 - Artículo

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AN - SCOPUS:85096348742

SN - 0022-149X

JO - Journal of Helminthology

JF - Journal of Helminthology

M1 - e210

ER -

A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

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