TY - JOUR
T1 - Addition of superoxide dismutase mimics during cooling process prevents oxidative stress and improves semen quality parameters in frozen/thawed ram spermatozoa
AU - Santiani, Alexei
AU - Evangelista, Shirley
AU - Sepúlveda, Néstor
AU - Risopatrón, Jennie
AU - Villegas, Juana
AU - Sánchez, Raúl
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - High levels of reactive oxygen species (ROS), which may be related to reduced semen quality, are detected during semen cryopreservation in some species. The objectives of this study were to measure the oxidative stress during ram semen cryopreservation and to evaluate the effect of adding 2 antioxidant mimics of superoxide dismutase (Tempo and Tempol) during the cooling process on sperm motility, viability, acrosomal integrity, capacitation status, ROS levels, and lipid peroxidation in frozen and/or thawed ram spermatozoa. Measuring of ROS levels during the cooling process at 35, 25, 15, and 5 °C and after freezing and/or thawing showed a directly proportional increase (P<0.05) when temperatures were lowering. Adding antioxidants at 10 °C confered a higher motility and sperm viability after cryopreservation in comparison with adding at 35 °C or at 35 °C/5 °C. After freezing and/or thawing, sperm motility was significantly higher (P<0.05) in Tempo and Tempol 1mM than that in control group. Percentage of capacitated spermatozoa was lower (P<0.05) in Tempo and Tempol 1mM in comparison with that in control group. In addition, ROS levels and lipid peroxidation in group Tempo 1mM were lower (P<0.05) than those in control group. These results demonstrate that ram spermatozoa are exposed to oxidative stress during the cooling process, specifically when maintained at 5 °C and that lipid peroxidation induced by high levels of ROS decreases sperm motility and induces premature sperm capacitation. In contrast, the addition of Tempo or Tempol at 0.5 to 1mM during the cooling process (10 °C) protects ram spermatozoa from oxidative stress.
AB - High levels of reactive oxygen species (ROS), which may be related to reduced semen quality, are detected during semen cryopreservation in some species. The objectives of this study were to measure the oxidative stress during ram semen cryopreservation and to evaluate the effect of adding 2 antioxidant mimics of superoxide dismutase (Tempo and Tempol) during the cooling process on sperm motility, viability, acrosomal integrity, capacitation status, ROS levels, and lipid peroxidation in frozen and/or thawed ram spermatozoa. Measuring of ROS levels during the cooling process at 35, 25, 15, and 5 °C and after freezing and/or thawing showed a directly proportional increase (P<0.05) when temperatures were lowering. Adding antioxidants at 10 °C confered a higher motility and sperm viability after cryopreservation in comparison with adding at 35 °C or at 35 °C/5 °C. After freezing and/or thawing, sperm motility was significantly higher (P<0.05) in Tempo and Tempol 1mM than that in control group. Percentage of capacitated spermatozoa was lower (P<0.05) in Tempo and Tempol 1mM in comparison with that in control group. In addition, ROS levels and lipid peroxidation in group Tempo 1mM were lower (P<0.05) than those in control group. These results demonstrate that ram spermatozoa are exposed to oxidative stress during the cooling process, specifically when maintained at 5 °C and that lipid peroxidation induced by high levels of ROS decreases sperm motility and induces premature sperm capacitation. In contrast, the addition of Tempo or Tempol at 0.5 to 1mM during the cooling process (10 °C) protects ram spermatozoa from oxidative stress.
KW - Cryopreservation
KW - Oxidative stress
KW - Ram sperm
KW - Superoxide dismutase
KW - Tempo
KW - Tempol
UR - http://www.scopus.com/inward/record.url?scp=84926164914&partnerID=8YFLogxK
U2 - 10.1016/j.theriogenology.2014.07.002
DO - 10.1016/j.theriogenology.2014.07.002
M3 - Artículo
C2 - 25108794
AN - SCOPUS:84926164914
SN - 0093-691X
VL - 82
SP - 884
EP - 889
JO - Theriogenology
JF - Theriogenology
IS - 6
ER -