Analysis of the genome sequence of an alpaca coronavirus

L. Jin, C. K. Cebra, R. J. Baker, D. E. Mattson, S. A. Cohen, Debora Elizabeth Alvarado Iparraguirre, G. F. Rohrmann

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

51 Citas (Scopus)

Resumen

Coronaviral infection of New World camelids was first identified in 1998 in llamas and alpacas with severe diarrhea. In order to understand this infection, one of the coronavirus isolates was sequenced and analyzed. It has a genome of 31,076 nt including the poly A tail at the 3′ end. This virus designated as ACoV-00-1381 (ACoV) encodes all 10 open reading frames (ORFs) characteristic of Group 2 bovine coronavirus (BCoV). Phylogenetic analysis showed that the ACoV genome is clustered closely (> 99.5% identity) with two BCoV strains, ENT and LUN, and was also closely related to other BCoV strains (Mebus, Quebec, DB2), a human corona virus (strain 043) (> 96%), and porcine hemagglutinating encephalomyelitis virus (> 93% identity). A total of 145 point mutations and one nucleotide deletion were found relative to the BCoV ENT. Most of the ORFs were highly conserved; however, the predicted spike protein (S) has 9 and 12 amino acid differences from BCoV LUN and ENT, respectively, and shows a higher relative number of changes than the other proteins. Phylogenetic analysis suggests that ACoV shares the same ancestor as BCoV ENT and LUN.

Idioma originalInglés
Páginas (desde-hasta)198-203
Número de páginas6
PublicaciónVirology
Volumen365
N.º1
DOI
EstadoPublicada - 15 ago 2007
Publicado de forma externa

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