Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte; C. Bonilla; G. Guimarães; R. A. Machado De Avila; T. M. Mendes; W. Silva; B. Tintaya; A. Yarleque; C. Chávez-Olórtegui

doi:10.1016/j.toxicon.2014.10.023

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte, C. Bonilla, G. Guimarães, R. A. Machado De Avila, T. M. Mendes, W. Silva, B. Tintaya, A. Yarleque, C. Chávez-Olórtegui

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20 Citas (Scopus)

Resumen

In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

Idioma original	Inglés
Páginas (desde-hasta)	37-40
Número de páginas	4
Publicación	Toxicon
Volumen	93
DOI	https://doi.org/10.1016/j.toxicon.2014.10.023
Estado	Publicada - ene. 2015

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Duarte, C. G., Bonilla, C., Guimarães, G., Machado De Avila, R. A., Mendes, T. M., Silva, W., Tintaya, B., Yarleque, A., & Chávez-Olórtegui, C. (2015). Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. Toxicon, 93, 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

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title = "Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru",

abstract = "In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.",

keywords = "Antivenom, Immunization, Loxosceles, Neutralization, Recombinant protein",

author = "Duarte, {C. G.} and C. Bonilla and G. Guimar{\~a}es and {Machado De Avila}, {R. A.} and Mendes, {T. M.} and W. Silva and B. Tintaya and A. Yarleque and C. Ch{\'a}vez-Ol{\'o}rtegui",

year = "2015",

month = jan,

doi = "10.1016/j.toxicon.2014.10.023",

language = "Ingl{\'e}s",

volume = "93",

pages = "37--40",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier Ltd.",

}

Duarte, CG, Bonilla, C, Guimarães, G, Machado De Avila, RA, Mendes, TM, Silva, W, Tintaya, B, Yarleque, A & Chávez-Olórtegui, C 2015, 'Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru', Toxicon, vol. 93, pp. 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

TY - JOUR

T1 - Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

AU - Duarte, C. G.

AU - Bonilla, C.

AU - Guimarães, G.

AU - Machado De Avila, R. A.

AU - Mendes, T. M.

AU - Silva, W.

AU - Tintaya, B.

AU - Yarleque, A.

AU - Chávez-Olórtegui, C.

PY - 2015/1

Y1 - 2015/1

N2 - In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

AB - In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

KW - Antivenom

KW - Immunization

KW - Loxosceles

KW - Neutralization

KW - Recombinant protein

UR - http://www.scopus.com/inward/record.url?scp=84919935913&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2014.10.023

DO - 10.1016/j.toxicon.2014.10.023

M3 - Artículo

C2 - 25448393

AN - SCOPUS:84919935913

SN - 0041-0101

VL - 93

SP - 37

EP - 40

JO - Toxicon

JF - Toxicon

ER -

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

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