TY - JOUR
T1 - Antimicrobial peptides purified from hydrolysates of kanihua (Chenopodium pallidicaule Aellen) seed protein fractions
AU - Moscoso-Mujica, Gladys
AU - Zavaleta, Amparo I.
AU - Mujica, Ángel
AU - Arnao, Inés
AU - Moscoso-Neira, Carlos
AU - Santos, Marco
AU - Sánchez, Johanna
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10/30
Y1 - 2021/10/30
N2 - The kanihua (Chenopodium pallidicaule Aellen) Andean grain from the Peruvian Altiplano presents proteins of 15% to 19%. The objective was to obtain purified bioactive antimicrobial peptides (AMPs), hydrolyzed with Alcalase and Pepsin-pancreatin sequential system of protein fractions of kanihua varieties Ramis (KR) and Cupi-Sayhua (KS), and hydrolysates with different degrees of hydrolysis (DH) and percentage inhibition (IP) of the growth of E. coli, S. aureus, and C. albicans. To obtain AMPs, nutraceuticals, bio-preservatives, and novel ingredients in food design. The results showed 216 hydrolysates (1%, w/v), only 28 presented significant difference compared to controls (IP ≥ 45%, p ≤ 0.05), 4 AMPs were purified by chromatography, glutelins KS 4 h (1:10) stood out with DH 40% and IP 52% and 70% of S. aureus and C. albicans, respectively (p ≤ 0.05), showed minimum inhibitory concentration (MIC) of 95% for E. coli (p ≤ 0.05), and presented an anionic charge. In conclusion, the simulated digestion in vitro showed higher DH (7%–67%) than Alcalase (13%–54%); the majority were extensive; of 28 hydrolysates with IP ≥ 45% 4 AMPs with important IPs were obtained, and one was anionic.
AB - The kanihua (Chenopodium pallidicaule Aellen) Andean grain from the Peruvian Altiplano presents proteins of 15% to 19%. The objective was to obtain purified bioactive antimicrobial peptides (AMPs), hydrolyzed with Alcalase and Pepsin-pancreatin sequential system of protein fractions of kanihua varieties Ramis (KR) and Cupi-Sayhua (KS), and hydrolysates with different degrees of hydrolysis (DH) and percentage inhibition (IP) of the growth of E. coli, S. aureus, and C. albicans. To obtain AMPs, nutraceuticals, bio-preservatives, and novel ingredients in food design. The results showed 216 hydrolysates (1%, w/v), only 28 presented significant difference compared to controls (IP ≥ 45%, p ≤ 0.05), 4 AMPs were purified by chromatography, glutelins KS 4 h (1:10) stood out with DH 40% and IP 52% and 70% of S. aureus and C. albicans, respectively (p ≤ 0.05), showed minimum inhibitory concentration (MIC) of 95% for E. coli (p ≤ 0.05), and presented an anionic charge. In conclusion, the simulated digestion in vitro showed higher DH (7%–67%) than Alcalase (13%–54%); the majority were extensive; of 28 hydrolysates with IP ≥ 45% 4 AMPs with important IPs were obtained, and one was anionic.
KW - Antimicrobial activity in percent inhibition
KW - Antimicrobial peptides
KW - Chenopodium pallidicaule Aellen
KW - Degree of hydrolysis
KW - Hydrolyzed
KW - Nutraceuticals
KW - Protein fractions
UR - http://www.scopus.com/inward/record.url?scp=85105554006&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2021.129951
DO - 10.1016/j.foodchem.2021.129951
M3 - Artículo
AN - SCOPUS:85105554006
SN - 0308-8146
VL - 360
JO - Food Chemistry
JF - Food Chemistry
M1 - 129951
ER -