Rol antioxidante de la l-carnitina en un modelo experimental de estrés oxidativo inducido por consumo elevado de fructosa

Marilin Maguiña-Alfaro, Silvia Suárez-Cunza, Luis Salcedo-Valdez, María Soberón-Lozano, Kelly Carbonel-Villanueva, Rosa Carrera-Palao

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Objectives: To evaluate the role of L-carnitine (LC) on fructose-induced oxidative stress in Holtzman rats. Materials and methods: An experimental study was carried out during 56 days, in patients assigned to 4 groups: Control, control+LC, fructose and fructose+LC. Patients in the fructose group received treatment during 56 days, and those in the LC groups were treated during the last 28 days. Fructose was given on demand and LC was administered orally at a dose of 500 g/kg/24 h. Lipid peroxidation (MDA), superoxide dismutase activity, free LC and mitochondrial and post-mitochondrial proteins were measured in liver tissue. Glycemia, insulin and the homeostasis model assessment of insulin resistance (HOMA-IR) were measured in blood plasma. We measured insulin concentration and studied the histology of pancreatic tissue. Results: LC treatment showed a decrease (p < 0.05) of MDA when compared to the control group (21.73 ± 5.36 nmol/g tissue vs. 64.46 ± 7.87 nmol/g tissue). Mitochondrial and post-mitochondrial proteins increased (p < 0.05) in comparison to the control group; pancreatic insulin also increased when compared to the control (341.8 ± 42.3 μUI/ml vs. 70.1 ± 9.6 μUI/ml, p<0.05). The role of LC against fructose-induced oxidative stress did not show any decrease of MDA, but decreased (p < 0.05) SOD Cu/Zn activity (9.39 ± 1.5 USOD/mg protein vs. 13.52 ± 1.5 USOD/mg protein). We observed that LC improves HOMA-IR in blood plasma. Histological analysis of the pancreas showed that the presence of LC increased the number and size of the islets of Langerhans. Conclusions: LC favors changes in the oxidative metabolism and it also contributes to glycemic homeostasis when fructose is consumed.

Título traducido de la contribuciónAntioxidant role of l-carnitine in an experimental model of oxidative stress induced by increased fructose consumption
Idioma originalEspañol
Páginas (desde-hasta)662-671
Número de páginas10
PublicaciónRevista Peruana de Medicina de Experimental y Salud Publica
Volumen37
N.º4
DOI
EstadoPublicada - 1 oct 2020

Nota bibliográfica

Publisher Copyright:
© 2020, Instituto Nacional de Salud. All rights reserved.

Palabras clave

  • Antioxidants
  • Fructose
  • Glycemia
  • Insulin
  • L-carnitine
  • Malondialdehyde Superoxide Dismutase
  • Oxidative stress

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