Caracterización básica y funcional del semen del Perro sin Pelo del Perúc

Alexei Santiani, María Pérez-Rosales, Carlos Challco

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm viability; 85.9 ± 5.1% high MMP; 83.6 ± 14.9% acrosomal integrity. These results represent the first report on the seminal characteristics of this breed and could be taken as reference values for a better selection of breeding males and for the diagnosis of reproductive alterations of the breed.

Título traducido de la contribuciónBasic and functional characterization of the Peruvian Hairless Dog semen
Idioma originalEspañol
Número de artículoe19251
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen31
N.º4
DOI
EstadoPublicada - 21 dic 2020

Nota bibliográfica

Publisher Copyright:
© 2020 Universidad Nacional Mayor de San Marcos. All rights reserved.

Palabras clave

  • Flow cytometry
  • Peruvian Hairless Dog
  • Semen
  • Spermatozoa

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