Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: Neutralization of its toxic effects by anti-loxoscelic antivenoms

G. Guimarães; C. Dias-Lopes; C. G. Duarte; L. Felicori; R. A. Machado de Avila; L. F.M. Figueiredo; J. de Moura; B. T. Faleiro; J. Barro; K. Flores; W. Silva; B. Tintaya; A. Yarleque; C. Bonilla; E. Kalapothakis; C. E. Salas; C. Chávez-Olortegui

doi:10.1016/j.toxicon.2013.04.018

Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: Neutralization of its toxic effects by anti-loxoscelic antivenoms

G. Guimarães, C. Dias-Lopes, C. G. Duarte, L. Felicori, R. A. Machado de Avila, L. F.M. Figueiredo, J. de Moura, B. T. Faleiro, J. Barro, K. Flores, W. Silva, B. Tintaya, A. Yarleque, C. Bonilla, E. Kalapothakis, C. E. Salas, C. Chávez-Olortegui

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Resumen

This manuscript describes the general biochemical properties and immunological characteristics of Peruvian spider Loxosceles laeta venom (PLlv), which is responsible for the largest number of accidents involving venomous animals in Peru. In this work, we observed that the venom of this spider is more lethal to mice when compared with L. laeta venom from Brazil (BLlv). The LD₅₀ of PLlv was 1.213mg/kg when the venom was intradermally injected. The venom displayed sphingomyelinase activity and produced dermonecrotic, hemorrhagic and edema effects in rabbits. 2-D SDS-PAGE separation of the soluble venoms resulted in a protein profile ranging from 20 to 205kDa. Anti-PLlv and anti-BLlv sera produced in rabbits and assayed by ELISA showed that rabbit antibodies cross-reacted with PLlv and BLlv and also with other Brazilian Loxosceles venoms. Western blotting analysis showed that bands corresponding to 25-35kDa are the proteins best recognized in every Loxosceles spp venoms analyzed. The immunized rabbits displayed protective effect after challenge with PLlv and BLlv. Invitro assays with horse anti-loxoscelic antivenoms produced in Brazil and Peru demonstrated that these commercial antivenoms were efficient to inhibit the sphingomyelinase activity of PLlv and BLlv.

Idioma original	Inglés
Páginas (desde-hasta)	90-97
Número de páginas	8
Publicación	Toxicon
Volumen	70
DOI	https://doi.org/10.1016/j.toxicon.2013.04.018
Estado	Publicada - ago. 2013

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Guimarães, G., Dias-Lopes, C., Duarte, C. G., Felicori, L., Machado de Avila, R. A., Figueiredo, L. F. M., de Moura, J., Faleiro, B. T., Barro, J., Flores, K., Silva, W., Tintaya, B., Yarleque, A., Bonilla, C., Kalapothakis, E., Salas, C. E., & Chávez-Olortegui, C. (2013). Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: Neutralization of its toxic effects by anti-loxoscelic antivenoms. Toxicon, 70, 90-97. https://doi.org/10.1016/j.toxicon.2013.04.018

@article{6f06d566dc3e4af1a3941c79cfdd9b95,

title = "Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: Neutralization of its toxic effects by anti-loxoscelic antivenoms",

abstract = "This manuscript describes the general biochemical properties and immunological characteristics of Peruvian spider Loxosceles laeta venom (PLlv), which is responsible for the largest number of accidents involving venomous animals in Peru. In this work, we observed that the venom of this spider is more lethal to mice when compared with L. laeta venom from Brazil (BLlv). The LD50 of PLlv was 1.213mg/kg when the venom was intradermally injected. The venom displayed sphingomyelinase activity and produced dermonecrotic, hemorrhagic and edema effects in rabbits. 2-D SDS-PAGE separation of the soluble venoms resulted in a protein profile ranging from 20 to 205kDa. Anti-PLlv and anti-BLlv sera produced in rabbits and assayed by ELISA showed that rabbit antibodies cross-reacted with PLlv and BLlv and also with other Brazilian Loxosceles venoms. Western blotting analysis showed that bands corresponding to 25-35kDa are the proteins best recognized in every Loxosceles spp venoms analyzed. The immunized rabbits displayed protective effect after challenge with PLlv and BLlv. Invitro assays with horse anti-loxoscelic antivenoms produced in Brazil and Peru demonstrated that these commercial antivenoms were efficient to inhibit the sphingomyelinase activity of PLlv and BLlv.",

keywords = "2-D electrophoresis, Anti-loxoscelic serum, Loxosceles laeta Spider venoms, Sphingomyelinases-D",

author = "G. Guimar{\~a}es and C. Dias-Lopes and Duarte, {C. G.} and L. Felicori and {Machado de Avila}, {R. A.} and Figueiredo, {L. F.M.} and {de Moura}, J. and Faleiro, {B. T.} and J. Barro and K. Flores and W. Silva and B. Tintaya and A. Yarleque and C. Bonilla and E. Kalapothakis and Salas, {C. E.} and C. Ch{\'a}vez-Olortegui",

note = "Funding Information: We would like to express gratitude to Dr. Marcelo Santoro for his critical review of this manuscript. This research was supported by Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior , Brazil – CAPES (Toxinologia no. 23038000825/2011-63 ), Funda{\c c}{\~a}o de Amparo a Pesquisa do Estado de Minas Gerais , Brazil (FAPEMIG) and by funds of the INCTTOX Program of Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico , Brazil (CNPq). The authors gratefully acknowledge the support and assistance of the Instituto Nacional de Salud, Peru. ",

year = "2013",

month = aug,

doi = "10.1016/j.toxicon.2013.04.018",

language = "Ingl{\'e}s",

volume = "70",

pages = "90--97",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier Ltd.",

}

Guimarães, G, Dias-Lopes, C, Duarte, CG, Felicori, L, Machado de Avila, RA, Figueiredo, LFM, de Moura, J, Faleiro, BT, Barro, J, Flores, K, Silva, W, Tintaya, B, Yarleque, A, Bonilla, C, Kalapothakis, E, Salas, CE & Chávez-Olortegui, C 2013, 'Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: Neutralization of its toxic effects by anti-loxoscelic antivenoms', Toxicon, vol. 70, pp. 90-97. https://doi.org/10.1016/j.toxicon.2013.04.018

TY - JOUR

T1 - Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom

T2 - Neutralization of its toxic effects by anti-loxoscelic antivenoms

AU - Guimarães, G.

AU - Dias-Lopes, C.

AU - Duarte, C. G.

AU - Felicori, L.

AU - Machado de Avila, R. A.

AU - Figueiredo, L. F.M.

AU - de Moura, J.

AU - Faleiro, B. T.

AU - Barro, J.

AU - Flores, K.

AU - Silva, W.

AU - Tintaya, B.

AU - Yarleque, A.

AU - Bonilla, C.

AU - Kalapothakis, E.

AU - Salas, C. E.

AU - Chávez-Olortegui, C.

N1 - Funding Information: We would like to express gratitude to Dr. Marcelo Santoro for his critical review of this manuscript. This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior , Brazil – CAPES (Toxinologia no. 23038000825/2011-63 ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais , Brazil (FAPEMIG) and by funds of the INCTTOX Program of Conselho Nacional de Desenvolvimento Científico e Tecnológico , Brazil (CNPq). The authors gratefully acknowledge the support and assistance of the Instituto Nacional de Salud, Peru.

PY - 2013/8

Y1 - 2013/8

N2 - This manuscript describes the general biochemical properties and immunological characteristics of Peruvian spider Loxosceles laeta venom (PLlv), which is responsible for the largest number of accidents involving venomous animals in Peru. In this work, we observed that the venom of this spider is more lethal to mice when compared with L. laeta venom from Brazil (BLlv). The LD50 of PLlv was 1.213mg/kg when the venom was intradermally injected. The venom displayed sphingomyelinase activity and produced dermonecrotic, hemorrhagic and edema effects in rabbits. 2-D SDS-PAGE separation of the soluble venoms resulted in a protein profile ranging from 20 to 205kDa. Anti-PLlv and anti-BLlv sera produced in rabbits and assayed by ELISA showed that rabbit antibodies cross-reacted with PLlv and BLlv and also with other Brazilian Loxosceles venoms. Western blotting analysis showed that bands corresponding to 25-35kDa are the proteins best recognized in every Loxosceles spp venoms analyzed. The immunized rabbits displayed protective effect after challenge with PLlv and BLlv. Invitro assays with horse anti-loxoscelic antivenoms produced in Brazil and Peru demonstrated that these commercial antivenoms were efficient to inhibit the sphingomyelinase activity of PLlv and BLlv.

AB - This manuscript describes the general biochemical properties and immunological characteristics of Peruvian spider Loxosceles laeta venom (PLlv), which is responsible for the largest number of accidents involving venomous animals in Peru. In this work, we observed that the venom of this spider is more lethal to mice when compared with L. laeta venom from Brazil (BLlv). The LD50 of PLlv was 1.213mg/kg when the venom was intradermally injected. The venom displayed sphingomyelinase activity and produced dermonecrotic, hemorrhagic and edema effects in rabbits. 2-D SDS-PAGE separation of the soluble venoms resulted in a protein profile ranging from 20 to 205kDa. Anti-PLlv and anti-BLlv sera produced in rabbits and assayed by ELISA showed that rabbit antibodies cross-reacted with PLlv and BLlv and also with other Brazilian Loxosceles venoms. Western blotting analysis showed that bands corresponding to 25-35kDa are the proteins best recognized in every Loxosceles spp venoms analyzed. The immunized rabbits displayed protective effect after challenge with PLlv and BLlv. Invitro assays with horse anti-loxoscelic antivenoms produced in Brazil and Peru demonstrated that these commercial antivenoms were efficient to inhibit the sphingomyelinase activity of PLlv and BLlv.

KW - 2-D electrophoresis

KW - Anti-loxoscelic serum

KW - Loxosceles laeta Spider venoms

KW - Sphingomyelinases-D

UR - http://www.scopus.com/inward/record.url?scp=84878212698&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2013.04.018

DO - 10.1016/j.toxicon.2013.04.018

M3 - Artículo

C2 - 23648420

AN - SCOPUS:84878212698

SN - 0041-0101

VL - 70

SP - 90

EP - 97

JO - Toxicon

JF - Toxicon

ER -

Biochemical and immunological characteristics of Peruvian Loxosceles laeta spider venom: Neutralization of its toxic effects by anti-loxoscelic antivenoms

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