Caracterización de las regiones organizadoras nucleolares en las células del tumor venéreo transmisible en caninos: Estudio histoquímico

The objective of the present study was to visualize and characterize the Nucleolar Organizer Regions (AgNORs) in Cells of Transmissible Venereal Tumor (TVT) in the canine through the impregnation with silver nitrate. One sample was collected at random from paraffin tissue of 30 canine tissue samples that were histological diagnosed (using Hematoxylin and Eosin stain) as TVT during the period of 2000-2006. The AgNORs were visualized and localized in 100 cells per sample. The average number of AgNORs per cell was 1.53, per cell nucleus was 90.91 and per nucleolus was 0.62. The distribution of AgNORs in the total number of cells was 60.7% (1821/ 3000) in the nucleus, 34.3% (1029/ 3000) in the nucleolus, and 5% (150/3000) in both structures.