TY - JOUR
T1 - Chemical composition and antibacterial effect of plantago major extract on periodontal pathogens
AU - Pesantes-Sangay, Sandra Jessenia
AU - Calla-Poma, Roger Damaso
AU - Requena-Mendizabal, Margarita Fé
AU - Alvino-Vales, María Isabel
AU - Millones-Gómez, Pablo Alejandro
N1 - Publisher Copyright:
© 2020, Association of Support to Oral Health Research (APESB). All rights reserved.
PY - 2020
Y1 - 2020
N2 - Objective: To determine the in vitro antibacterial effect of different concentrations of the ethanol extract of Plantago major (plantain) on Porphyromonas gingivalis and Fusobacterium nucleatum. Material and Methods: Bacterial susceptibility tests were used in conjunction with the agar diffusion test and the minimum inhibitory concentration (MIC) test using the broth macrodilution technique. Results: Different concentrations of ethanol extract (25%, 50%, 75% and 100%) dissolved in 70% ethanol were used, with a positive control (0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride) and a negative control (70% alcohol). The extracts at 75% and 100% showed inhibition halos against both strains studied. With 0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride, inhibition halos averaged 14.9 mm, in contrast to 70° alcohol, where no bacterial inhibition was observed. The MIC was 50% for both species. Conclusion: The ethanol extract of Plantago major presents an in vitro antibacterial effect on Porphyromonas gingivalis, they may have potential applications in food and pharmaceutical products.
AB - Objective: To determine the in vitro antibacterial effect of different concentrations of the ethanol extract of Plantago major (plantain) on Porphyromonas gingivalis and Fusobacterium nucleatum. Material and Methods: Bacterial susceptibility tests were used in conjunction with the agar diffusion test and the minimum inhibitory concentration (MIC) test using the broth macrodilution technique. Results: Different concentrations of ethanol extract (25%, 50%, 75% and 100%) dissolved in 70% ethanol were used, with a positive control (0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride) and a negative control (70% alcohol). The extracts at 75% and 100% showed inhibition halos against both strains studied. With 0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride, inhibition halos averaged 14.9 mm, in contrast to 70° alcohol, where no bacterial inhibition was observed. The MIC was 50% for both species. Conclusion: The ethanol extract of Plantago major presents an in vitro antibacterial effect on Porphyromonas gingivalis, they may have potential applications in food and pharmaceutical products.
KW - Gram-Negative Bacteria
KW - Microbiology
KW - Periodontics
KW - Plants, Medicinal
UR - http://www.scopus.com/inward/record.url?scp=85088601518&partnerID=8YFLogxK
U2 - 10.1590/pboci.2020.100
DO - 10.1590/pboci.2020.100
M3 - Artículo
AN - SCOPUS:85088601518
SN - 1519-0501
VL - 20
SP - 1
EP - 10
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
M1 - e0012
ER -