A western blot assay to detect antibodies against Sarcocystis aucheniae (SA) from alpacas was developed for in vivo diagnosis of sarcocystiosis in domestic camelids. The antigen consists of a soluble extract obtained from SA macrocysts (MSA) of naturally infected alpacas. A SDS-PAGE (15%) electrophoretic analysis revealed fifteen peptides at 25-127 kDa. Western blot assay ofsera from MSA immunized rabbits detected three peptides at 58-50 KDa, while the sera from SA diagnosed alpacas reacted against four protein bands. Sera from neonate alpacas and from non-SA infected alpacas did not display reactivity to the MSA peptides. Specific immune reactivity ofthe alpaca antibodies against SA with four MSA peptides by a western blot assay has been documented.
|Título traducido de la contribución||Development of a Western blot method for the detection of antibodies against Sarcocystis aucheniae in alpacas|
|Número de páginas||4|
|Publicación||Revista de Investigaciones Veterinarias del Peru|
|Estado||Publicada - 1999|
|Publicado de forma externa||Sí|
- Western blot