Detección del gen PrP de scrapie en ovinos junín

Rivera J. Kamilo, Maturrano H. Lenin, Manuel Aguilar Juan, Rosadio A. Raúl

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The aim of this study was to establish the frequency of resistance and susceptible genotypes to scrapie in the Junin sheep breed. For this, 56 blood samples were collected in a sheep farm in the central highlands of Peru. The DNA was extracted and the PCR products were analyzed by enzymatic digestion using restriction endonucleases that recognize the PrP gene polymorphisms. Four of the 5 haplotypes reported for the PrP gene were identified: ARR (48.2%), ARQ (35.7%), AHQ (10.7%) and VRQ (5.4%). In addition, 7 of the 15 reported genotypes for the Prion gene were found: ARR/ARR (17.9%), ARR/AHQ (10.7%), ARR/ARQ (42.9%), ARQ/AHQ (10.7%), ARQ/ARQ (7.1%), ARR/ VRQ (7.1%) and ARQ/VRQ (3.6%). Finally, 82.1% of the animals had genotypes resistant to the disease and 17.9% showed genotypes of susceptibility.

Título traducido de la contribuciónDetection of the PrP gene in junin sheep
Idioma originalEspañol
Páginas (desde-hasta)57-65
Número de páginas9
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen26
N.º1
DOI
EstadoPublicada - 2015
Publicado de forma externa

Palabras clave

  • Junin sheep
  • Prion gene
  • Scrapie

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