Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel; Sukwan Handali; Edneia C. Bueno; Azra Khan; Kathy Hancock; Armando E. Gonzalez; Hector H. Garcia; Robert H. Gilman; Victor C.W. Tsang

doi:10.1080/15321810600573028

Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel, Sukwan Handali, Edneia C. Bueno, Azra Khan, Kathy Hancock, Armando E. Gonzalez, Hector H. Garcia, Robert H. Gilman, Victor C.W. Tsang

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4 Citas (Scopus)

Resumen

Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development. Copyright © Taylor & Francis Group, LLC.

Idioma original	Inglés estadounidense
Páginas (desde-hasta)	173-181
Número de páginas	9
Publicación	Journal of Immunoassay and Immunochemistry
DOI	https://doi.org/10.1080/15321810600573028
Estado	Publicada - 1 jul. 2006

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Scheel, C. M., Handali, S., Bueno, E. C., Khan, A., Hancock, K., Gonzalez, A. E., Garcia, H. H., Gilman, R. H., & Tsang, V. C. W. (2006). Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy. Journal of Immunoassay and Immunochemistry, 173-181. https://doi.org/10.1080/15321810600573028

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abstract = "Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development. Copyright {\textcopyright} Taylor & Francis Group, LLC.",

author = "Scheel, {Christina M.} and Sukwan Handali and Bueno, {Edneia C.} and Azra Khan and Kathy Hancock and Gonzalez, {Armando E.} and Garcia, {Hector H.} and Gilman, {Robert H.} and Tsang, {Victor C.W.}",

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AU - Khan, Azra

AU - Hancock, Kathy

AU - Gonzalez, Armando E.

AU - Garcia, Hector H.

AU - Gilman, Robert H.

AU - Tsang, Victor C.W.

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AB - Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development. Copyright © Taylor & Francis Group, LLC.

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Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

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