Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom

Henri Bailon Calderon, Verónica Olga Yaniro Coronel, Omar Alberto Cáceres Rey, Elizabeth Gaby Colque Alave, Walter Jhon Leiva Duran, Carlos Padilla Rojas, Harrison Montejo Arevalo, David García Neyra, Marco Galarza Pérez, César Bonilla, Benigno Tintaya, Giulia Ricciardi, Natalia Smiejkowska, Ema Romão, Cécile Vincke, Juan Lévano, Mary Celys, Bruno Lomonte, Serge Muyldermans

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

Snake envenoming is a globally neglected public health problem. Antivenoms produced using animal hyperimmune plasma remain the standard therapy for snakebites. Although effective against systemic effects, conventional antivenoms have limited efficacy against local tissue damage. In addition, potential hypersensitivity reactions, high costs for animal maintenance, and difficulties in obtaining batch-to-batch homogeneity are some of the factors that have motivated the search for innovative and improved therapeutic products against such envenoming. In this study, we have developed a set of nanobodies (recombinant single-domain antigen-binding fragments from camelid heavy chain-only antibodies) against Bothrops atrox snake venom hemorrhagic and myotoxic components. An immune library was constructed after immunizing a Lama glama with whole venom of B. atrox, from which nanobodies were selected by phage display using partially purified hemorrhagic and myotoxic proteins. Biopanning selections retrieved 18 and eight different nanobodies against the hemorrhagic and the myotoxic proteins, respectively. In vivo assays in mice showed that five nanobodies inhibited the hemorrhagic activity of the proteins; three neutralized the hemorrhagic activity of whole B. atrox venom, while four nanobodies inhibited the myotoxic protein. A mixture of the anti-hemorrhagic and anti-myotoxic nanobodies neutralized the local tissue hemorrhage and myonecrosis induced by the whole venom, although the nanobody mixture failed to prevent the venom lethality. Nevertheless, our results demonstrate the efficacy and usefulness of these nanobodies to neutralize important pathologies of the venom, highlighting their potential as innovative therapeutic agents against envenoming by B. atrox, a viperid species causing many casualties in South America.

Idioma originalInglés
Número de artículo655
PublicaciónFrontiers in Immunology
Volumen11
DOI
EstadoPublicada - 7 may 2020
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© Copyright © 2020 Bailon Calderon, Yaniro Coronel, Cáceres Rey, Colque Alave, Leiva Duran, Padilla Rojas, Montejo Arevalo, García Neyra, Galarza Pérez, Bonilla, Tintaya, Ricciardi, Smiejkowska, Romão, Vincke, Lévano, Celys, Lomonte and Muyldermans.

Huella

Profundice en los temas de investigación de 'Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom'. En conjunto forman una huella única.

Citar esto