TY - JOUR
T1 - Distribution of tumor-infiltrating immune cells in glioblastoma
AU - Orrego, Enrique
AU - Castaneda, Carlos A.
AU - Castillo, Miluska
AU - Bernabe, Luis A.
AU - Casavilca, Sandro
AU - Chakravarti, Arnab
AU - Meng, Wei
AU - Garcia-Corrochano, Pamela
AU - Villa-Robles, Maria R.
AU - Zevallos, Rocio
AU - Mejia, Omar
AU - Deza, Pedro
AU - Belmar-Lopez, Carolina
AU - Ojeda, Luis
N1 - Publisher Copyright:
© 2018 2018 Orrego, Castaneda, Castillo.
PY - 2018
Y1 - 2018
N2 - Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.
AB - Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.
KW - MGMT
KW - biomarker
KW - glioblastoma
KW - macrophages
KW - overall survival
KW - prognosis
KW - tumor-infiltrating lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=85062250676&partnerID=8YFLogxK
U2 - 10.2217/cns-2017-0037
DO - 10.2217/cns-2017-0037
M3 - Artículo
C2 - 30299157
AN - SCOPUS:85062250676
SN - 2045-0907
VL - 7
JO - CNS oncology
JF - CNS oncology
IS - 4
M1 - 0037
ER -