The aim of this study was to compare the relative expression levels of the gene from exon 1 of the IgA in the intestinal epithelium of baby alpacas untreated and treated orally with Clostridium perfringens antigens and all-trans retinoic acid (ATRA). Thirty two animals were sampled: 14 treated (6 of 1 day old and 8 between 7-14 days old) and 18 untreated (10 of 1 day old and 8 between 7-14 days old). The animals were slaughtered after one week of the treatment and 2 cm of the jejunum was collected and kept at-196 °C. Total RNA was extracted from each sample using the TRIzol® method and complementary DNA (cDNA) was synthesized. PCR and RT-PCR Real Time were conducted using specific oligonucleotides designed for the detection of exon 1 of the region Fc IgA in the alpaca. The quantification of the relative expression by normalization with GAPDH gene mRNA and the relative expression calculations were performed using the comparative Ct method (2-δδCt). The results showed that the administration of C. perfringens antigens and ATRA exerts positive immunomodulatory effect of IgA on the intestinal mucosa of treated baby alpacas. Animals of both age groups showed higher IgA mRNA production with respect the non-treated animals (p<0.05).

Título traducido de la contribuciónEffect of clostridium perfringens antigens and acid retinoic on iga expression in the intestinal mucosa of newborn alpacas (vicugna pacos)
Idioma originalEspañol
Páginas (desde-hasta)689-697
Número de páginas9
PublicaciónRevista de Investigaciones Veterinarias del Peru
EstadoPublicada - 2015

Palabras clave

  • Clostridium perfringens
  • Immunoglobulin A
  • Real Time RT-PCR
  • Relative quantification
  • Retinoic acid
  • South American camelids


Profundice en los temas de investigación de 'Efecto de Antígenos de Clostridium perfringens y Ácido Retinoico sobre la Expresión de IgA en la Mucosa Intestinal de Crías de Alpacas (Vicugna pacos)'. En conjunto forman una huella única.

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