Resumen

Objectives. To evaluate the effect of ZnO, TiO2 and SiO2 nanoparticles on cell viability and expression of the interleukin 7, interleukin 3, and granulocyte-macrophage colony stimulating factor (GM-CSF) genes in Mus musculus. Materials and methods. Red bone marrow was extracted from five Balb/c mice for the analysis of cell viability using the MTT test. The mice were divided into two groups of five each: one group was inoculated intraperitoneally with 0.5, 1.0, 2.5, 5.0, and 10 mg/kg of ZnO and SiO2 nanoparticles, respectively, and the other group was inoculated with 5.0, 10.0, 15.0, 20.0, and 25 mg/kg of TiO2 nanoparticles, respectively. Thirty hours later, RNA was extracted from the red bone marrow of the mice in both groups for gene expression analysis using quantitative PCR and RT-PCR. Results. ZnO and SiO2 nanoparticles reduced cell viability in a dose-dependent manner by 37% and 26%, respectively, starting at a dose of 1 mg/kg. TiO2 nanoparticles at 5 mg/kg and 10 mg/kg reduced the gene expression of interleukins 7 and 3 by 55.3% and 70.2%, respectively, and SiO2 nanoparticles caused the greatest decrease (91%) in the expression of GM-CSF. ZnO nanoparticles reduced the expression of GM-CSF starting at doses of 20 mg/kg and 25 mg/kg. Conclusions: ZnO, SiO2 and TiO2 nanoparticles affect cell viability and gene expression in the mouse bone marrow.

Título traducido de la contribuciónEffect of the industrial nanoparticles TiO2, SiO2 and ZnO on cell viability and gene expression in red bone marrow of mus musculus
Idioma originalEspañol
Páginas (desde-hasta)436-444
Número de páginas9
PublicaciónRevista Peruana de Medicina de Experimental y Salud Publica
Volumen34
N.º3
DOI
EstadoPublicada - 1 jul 2017

Nota bibliográfica

Publisher Copyright:
© 2017, Instituto Nacional de Salud. All rights reserved.

Palabras clave

  • Genic expression
  • Interleukins
  • Mus musculus
  • Nanoparticles

Huella

Profundice en los temas de investigación de 'Efecto de las nanopartículas industriales TiO<sub>2</sub>, SiO<sub>2</sub> y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de mus musculus'. En conjunto forman una huella única.

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