Evaluación de la prueba de inhibición de la hemaglutinación vs. Elisa para la detección de anticuerpos contra mycoplasma gallisepticum y mycoplasma synoviae

The hemagglutination inhibition test (HI) and an indirect ELISA were used to detect antibodies against Mycoplasma gallisepticum (MG) and M. synoviae (MS) in 102 commercial layer hens from Chincha Province, lea Department, Peru. The HI test detected MG antibodies in 20% ofthe layers tested, whereas ELISA detectedits presence in 52%. The frequency of MS reactors detected by HI was 5% compared to 95% for ELISA. A polymerase chain reaction (PCR) was set upas a reference test to estimate the sensitivity, specificity, positive and negative prediction values for the HI and ELISA serological tests. The ELISA test was found to be more sensitive for detection of antibodies to MG (67%) and MS (97%) than the HI test which detected low levels ofMG (12%) and MS (5.3%). The specificity recorded for ELISA was 68% to MG and 25% to MS, while the figures for HI were 70.4% and 100%. The Kappa Index test demonstrates that HI and ELISA produce conflicting results for the diagnosis ofMG and MS, and the McNemar test shows that HI can not reliably replace ELISA in this task.