Expression of candidate cold stress and metabolic related genes in Acidithiobacillus ferrivorans PQ33 strain using ferrous iron as electron donor

Gregory Guerra-Bieberach, Robert Ccorahua-Santo, Anika Eca, Jordan Bernaldo, Tito Sánchez, Chachi Rojas-Ayala, Pablo Ramirez

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The identification of genes involved in cold adaptations of psychrotolerant bacteria Acidithiobacillus ferrivorans is important for biomining processes that take place at low temperatures like Andean mining installations in Peru. We have performed relative quantification RT-qPCR on candidate genes to have a role in adaptations at low temperature (5°C). The candidate genes analyzed were six: Two trehalose synthesis pathway genes, trehalose synthase (treS) and malto-oligosiltrehalose trehalohydrolase (treZ) showing no overexpression at 5°C. Two diguanylate cyclases genes related to exopolymer synthesis and biofilm formation (designated as dgc-I and dgc-II in this paper) were overexpressed at 21°C. The rusA and rusB genes involved in iron oxidation showed no significant change for rusA and no expression for rusB gene in any of both conditions. Genes rpoC, gyrB and alaS were validated as reference genes. These results show congruency with trancriptomics studies about gene expression of A. ferrivorans. Furthermore, the trehalose synthesis genes show no overexpression at low temperatures suggesting that other cold adaptation mechanisms are involved.

Idioma originalInglés
Título de la publicación alojada22nd International Biohydrometallurgy Symposium
EditoresSabrina Hedrich, Axel Schippers, Kathrin Rubberdt, Franz Glombitza, Wolfgang Sand, Wolfgang Sand, Mario Vera Veliz, Sabine Willscher
EditorialTrans Tech Publications Ltd
Número de páginas4
ISBN (versión impresa)9783035711806
EstadoPublicada - 2017
Evento22nd International Biohydrometallurgy Symposium, 2017 - Freiberg, Alemania
Duración: 24 set. 201727 set. 2017

Serie de la publicación

NombreSolid State Phenomena
Volumen262 SSP
ISSN (versión impresa)1012-0394
ISSN (versión digital)1662-9779


Conferencia22nd International Biohydrometallurgy Symposium, 2017

Nota bibliográfica

Publisher Copyright:
© 2017 Trans Tech Publications, Switzerland.


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