TY - JOUR
T1 - Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru
AU - Patel, Nisha B.
AU - Tito, Raul Y.
AU - Obregón-Tito, Alexandra J.
AU - O'Neal, Lindsey
AU - Trujillo-Villaroel, Omar
AU - Marin-Reyes, Luis
AU - Troncoso-Corzo, Luzmila
AU - Guija-Poma, Emilio
AU - Hamada, Moriyuki
AU - Uchino, Yoshihito
AU - Lewis, Cecil M.
AU - Lawson, Paul A.
N1 - Publisher Copyright:
© 2014.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T).
AB - A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T).
KW - 16S rRNA-phylogeny
KW - BD
KW - CCUG
KW - Ezakiella peruensis gen. nov., sp. nov.
KW - GPAC
KW - HMP
KW - NBRC
KW - PY
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=84949117167&partnerID=8YFLogxK
U2 - 10.1016/j.anaerobe.2014.12.002
DO - 10.1016/j.anaerobe.2014.12.002
M3 - Artículo
C2 - 25481562
AN - SCOPUS:84949117167
SN - 1075-9964
VL - 32
SP - 43
EP - 48
JO - Anaerobe
JF - Anaerobe
ER -